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Effects of Chronic Beta-Alanine Supplementation with and without Acute Sodium Bicarbonate Ingestion on Peak Muscle Carnosine Concentration, Washout and High-Intensity Exercise Performance

Grant number: 11/19513-2
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): November 01, 2012
Effective date (End): October 31, 2015
Field of knowledge:Health Sciences - Physical Education
Principal Investigator:Bruno Gualano
Grantee:Bryan Saunders
Host Institution: Escola de Educação Física e Esporte (EEFE). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Carnosine (beta-alanyl-L-histidine) is a cytoplasmic dipeptide found in high concentrations in the skeletal muscle of both vertebrates and non-vertebrates, as well as in the central nervous system. It has been shown that the elevation of muscle carnosine concentrations through the dietary intake of carnosine or supplementation with beta-alanine provides a method of increasing intracellular buffering capacity during exercise. Additionally, one study to date has indicated that sodium bicarbonate may have a synergistic effect on performance when co-supplemented with beta-alanine. Thus, the aim of this study is to examine the effects of beta-alanine supplementation with and without acute sodium bicarbonate supplementation on: a) peak muscle carnosine concentration; b) muscle carnosine washout and c) high-intensity exercise performance. Forty-five physically active male volunteers (aged between 18-30 years) will be recruited to this study. Subjects will be randomly allocated to one of three supplementation groups: 1) beta-alanine group receiving 6.4 g/d for 16 weeks followed by a maltodextrin placebo for 16 weeks; 2) beta-alanine supplementation group receiving 3.2 g/d for 32 weeks; 3) Placebo group receiving a maltodextrin placebo for 32 weeks. At weeks 0, 8, 16, 24 and 32, muscle carnosine content will be assessed in vivo by 1H-MRS and high-intensity exercise capacity will be determined using the CCT110% test. One of the tests will be performed following the acute ingestion of 0.3 g/kg sodium bicarbonate taken over the preceding 4 h and the other will be following the acute ingestion of a matched placebo (maltodextrin). Blood samples will be analysed for lactate, blood gases, haemoglobin and pH. After the normality and homogeneity of the variance are confirmed, the dependent variables will be compared using a mixed model. The significance level will be set at p < 0.05.

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SAUNDERS, B.; DE OLIVEIRA, L. F.; DA SILVA, R. P.; DE SALLES PAINELLI, V.; GONCALVES, L. S.; YAMAGUCHI, G.; MUTTI, T.; MACIEL, E.; ROSCHEL, H.; ARTIOLI, G. G.; et al. Placebo in sports nutrition: a proof-of-principle study involving caffeine supplementation. SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS, v. 27, n. 11, p. 1240-1247, . (14/11948-8, 11/19513-2, 13/14746-4, 13/04806-0)
GONCALVES, LIVIA DE SOUZA; PAINELLI, VITOR DE SALLES; YAMAGUCHI, GUILHERME; DE OLIVEIRA, LUANA FARIAS; SAUNDERS, BRYAN; DA SILVA, RAFAEL PIRES; MACIEL, ERIKA; ARTIOLI, GUILHERME GIANNINI; ROSCHEL, HAMILTON; GUALANO, BRUNO. Dispelling the myth that habitual caffeine consumption influences the performance response to acute caffeine supplementation. Journal of Applied Physiology, v. 123, n. 1, p. 213-220, . (11/19513-2, 13/14746-4, 12/13026-5, 13/04806-0)
PAULO LOPES-SILVA, JOAO; DA SILVA SANTOS, JONATAS FERREIRA; MAGNANI BRANCO, BRAULIO HENRIQUE; CAL ABAD, CESAR CAVINATO; DE OLIVEIRA, LUANA FARIAS; LOTURCO, IRINEU; FRANCHINI, EMERSON. Caffeine Ingestion Increases Estimated Glycolytic Metabolism during Taekwondo Combat Simulation but Does Not Improve Performance or Parasympathetic Reactivation. PLoS One, v. 10, n. 11, . (11/19513-2)
DE ARAUJO DIAS, GABRIELA FROIO; SILVA, VINICIUS DA EIRA; PAINELLI, VITOR DE SALLES; SALE, CRAIG; ARTIOLI, GUILHERME GIANNINI; GUALANO, BRUNO; SAUNDERS, BRYAN. (In) Consistencies in Responses to Sodium Bicarbonate Supplementation: A Randomised, Repeated Measures, Counterbalanced and Double-Blind Study. PLoS One, v. 10, n. 11, . (14/11948-8, 11/19513-2, 13/14746-4, 13/04806-0)
SAUNDERS, BRYAN; PAINELLI, VITOR DE SALLES; DE OLIVEIRA, LUANA FARIAS; SILVA, VINICIUS DA EIRA; DA SILVA, RAFAEL PIRES; RIANI, LUIZ; FRANCHI, MARIANA; GONCALVES, LIVIA DE SOUZA; HARRIS, ROGER CHARLES; ROSCHEL, HAMILTON; et al. Twenty-four Weeks of beta-Alanine Supplementation on Carnosine Content, Related Genes, and Exercise. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 49, n. 5, p. 896-906, . (13/14746-4, 14/11948-8, 12/13026-5, 11/19513-2, 13/04806-0, 15/22686-7)

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