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Qualitative and quantitative evaluation of the remineralizing potential of different fluoride varnishes in white spot lesions produced in vitro in primary teeth

Grant number: 12/03036-3
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): June 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Fernanda Miori Pascon
Grantee:Thais Varanda
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil


The early childhood caries (ECC) is an oral health problem in Brazil, most common in children in preschool. It can be related with a multifactorial etiology and can develop rapidly, causing serious health problems in children and even the quality of life for them. White spot lesions are commonly observed in children and, when diagnosed early and treated further at this stage is more favorable for the patient. The enamel remineralization has been studied and many agents have been evaluated. However, different types of fluoride releasing dental materials and new technologies are available on the market, including toothpaste, sealants, glass ionomer cement and varnishes. Therefore, the aim of this study is to evaluate the remineralizing potential of three varnishes: Duraphat® - 5% sodium fluoride varnish - Colgate; MI " Varnish - 5% sodium fluoride varnish with Recaldent" (CPP-ACP)- GC America Inc., and Varnish " - 5% sodium fluoride varnish with Tri-Calcium Phosphate (TCP)-3M ESPE, in white spot lesions of primary teeth produced in vitro. The sample will consist of 80 extracted primary teeth and the response variables will be: (1) the depth of the lesion artificially formed, (2) the depth of remineralization, both measured by Polarized Light Microscopy, (3) percentage mineral evaluated by Knoop microhardness test, (4) the morphology of enamel after remineralization evaluated by scanning electron and polarized light microcopies and (5) the amount of calcium and phosphorus, assessed by spectroscopy energy dispersive X-ray. The results will be tested for normality and after that the appropriate statistical tests will be applied separately, considering the factors under study. For the morphological analysis (qualitative analysis) observed in scanning electron microscopy and polarized light microscopy, the images will be submitted to descriptive analysis. Analyzes will be performed considering the significance level of 5%.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOARES-YOSHIKAWA, ALINE LAIGNIER; VARANDA, THAIS; IWAMOTO, ALEXSANDRA SHIZUE; KANTOVITZ, KAMILA ROSAMILIA; PUPPIN-RONTANI, REGINA MARIA; PASCON, FERNANDA MIORI. Fluoride release and remineralizing potential of varnishes in early caries lesions in primary teeth. MICROSCOPY RESEARCH AND TECHNIQUE, v. 84, n. 5, . (12/03036-3)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
VARANDA, Thais. Qualitative and quantitative evaluation of the formation and treatment of white spot lesions produced in vitro in teeth deciduous. 2014. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.

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