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In vitro study of the microneedles system appicability for topical anesthesia in swine oral tissues.

Grant number: 12/02492-5
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2012
Effective date (End): February 29, 2016
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Michelle Franz Montan Braga Leite
Grantee:Luciano Serpe
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil


Recent studies have demonstrated the use of microneedles for increasing skin topical anesthesia and absorption of topically applied drugs aiming at systemic action. The objective of the use of microneedles is to cross the stratum corneum painlessly, improving the absorption of drugs in topical route administration. This system could be useful for improving topical anesthesia efficacy in dentistry. Thus, the aim of the present study is to evaluate the applicability of the microneedle system in vitro in swine oral tissues aiming at future use in vivo in volunteers. The following analysis will be performed: histological characterization of micropores created on palatal mucosa by light microscopy and confocal microscopy following mucosal perforation with different microneedle sizes (0.2, 0.5 and 1 mm); and evaluation of the micropores (created by different microneedles sizes described previously) influence in the permeation parameters and retention of a topical anesthetic formulation. The in vitro permeation and retention studies will be performed at Franz type diffusion cells with the topical anesthetic EMLA® (AstraZeneca - Brazil). After permeation experiments, retention of local anesthetics into the tissue will be analyzed. Quantification of local anesthetics in permeation and retention studies will be performed by High Performance Liquid Chromatography (HPLC). The microneedle system that will to be used in the present study is the Dermaroller® (Microagulhas Dermaroller® - Dermaroller Deutchland S.A.R.L. Alemanha).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SERPE, LUCIANO; MUNIZ, BRUNO VILELA; DOS SANTOS, CLEITON PITA; DA SILVA, CAMILA BATISTA; VOLPATO, MARIA CRISTINA; GROPPO, FRANCISCO CARLOS; VIANNA LOPEZ, RENATA FONSECA; FRANZ-MONTAN, MICHELLE. Full-Thickness Intraoral Mucosa Barrier Models for In Vitro Drug-Permeation Studies Using Microneedles. Journal of Pharmaceutical Sciences, v. 108, n. 5, p. 1756-1764, . (12/06974-4, 12/02492-5, 11/18113-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SERPE, Luciano. Standardization of oral mucosa barrier models, experimental conditions and use of microneedles as permeation enhancer for in vitro permeation studies. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.

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