Dendritic cells pulsed with peptide 10 (P10) of Paracoccidioides brasiliensis in the control of experimental paracoccidioidomycosis. Reversal of the anergic state and control of acute infection.

Abstract

Paracoccidiodomycosis (PCM), a prevailing systemic mycosis in Latin America, is a granulomatous disease caused by the thermo-dimorphic fungus Paracoccidioides brasiliensis. In Brazil, PMC is ranked as the tenth cause of death among the chronic infectious and parasitic diseases. Dendritic cells are the most efficient antigen presenting cells. When used as adjuvant these cells are at least 100-1000 times more fficient presenting peptides than a non specific adjuvant. The P10 peptide is a specific sequence containing 15 amino acids derived from gp43 secreted by the fungus. It is ecognized by T cells and is capable to induce a Th1 response that protects mice in a murine infection model of the disease.In this sense, this paper aims to examine the therapeutic effect of dendritic cells pulsed with P10 peptide in B10.A mice, BALB / c, A / J and C57/BL6; check for immunohistochemistry, the activity of dendritic cells pulsed with P10 in the recruitment of other cell populations in the lungs of infected mice, to evaluate the ability to reverse the anergic state in mice infected with P. and P. brasiliensis lutzii and verify the production of proinflammatory cytokines and lung structure of mice infected and immunized with dendritic cells pulsed with peptide P10.