Biotechnology of marine natural products has been growing in recent decades and gained prominence in the international arena in cutting-edge research for numerous areas of science such as pharmacology, medicine, food industry cosmetology. Based on this broad field that requires much further research, our group aims to evaluate the effect of sulfated polysaccharides extracted from seaweeds on biological activity, structural, pharmacological of secretory and cytosolic phospholipase A2, pro-inflammatory studies using as model the PLA2 isolated from the venom of rattlesnake that has structural similarity with the human PLA2. Thus, we will develop and refine protocols for purification of polysaccharides from macroalgae (Rodophyta and Clorophita). The effect of molecular interaction of different sulfated polysaccharides will be made by means of molecular exclusion HPLC and ion exchange in addition to monitoring by spectroscopic UV-Vis, Fluorescence, and Circular Dichroism. The effect of molecular interaction of sPLA2 with different sulfated polysaccharides on the enzymatic activity of sPLA2 is evaluated by using synthetic substrates specific for sPLA2. Thus, we will determine the inhibitory concentration of sulfated polysaccharides on the enzymatic activity of sPLA2, effects on the constants Vmax and Km Finally, using the models of paw edema, swelling of skin and anti-inflammatory activity of sulfated polysaccharides In addition, we intend to evaluate the effects of these polysaccharides against myotoxicity and aggregating activity of sPLA2.
News published in Agência FAPESP Newsletter about the scholarship: