Advanced search
Start date
Betweenand
Related content

CHARACTERIZATION OF THE EFFECTS OF SEAWEED POLYSACCHARIDES SULFATE ON THE FUNCTION OF BIOLOGICAL AND PHARMACOLOGICAL sPLA2 PRO INFLAMMATORY ISOLATED TOTAL POISON Crotalus durissus terrificus

Grant number: 11/14241-4
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): May 01, 2012
Effective date (End): September 30, 2013
Field of knowledge:Interdisciplinary Subjects
Principal Investigator:Marcos Hikari Toyama
Grantee:Camila Lehnhardt Pires
Host Institution: Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil

Abstract

Biotechnology of marine natural products has been growing in recent decades and gained prominence in the international arena in cutting-edge research for numerous areas of science such as pharmacology, medicine, food industry cosmetology. Based on this broad field that requires much further research, our group aims to evaluate the effect of sulfated polysaccharides extracted from seaweeds on biological activity, structural, pharmacological of secretory and cytosolic phospholipase A2, pro-inflammatory studies using as model the PLA2 isolated from the venom of rattlesnake that has structural similarity with the human PLA2. Thus, we will develop and refine protocols for purification of polysaccharides from macroalgae (Rodophyta and Clorophita). The effect of molecular interaction of different sulfated polysaccharides will be made by means of molecular exclusion HPLC and ion exchange in addition to monitoring by spectroscopic UV-Vis, Fluorescence, and Circular Dichroism. The effect of molecular interaction of sPLA2 with different sulfated polysaccharides on the enzymatic activity of sPLA2 is evaluated by using synthetic substrates specific for sPLA2. Thus, we will determine the inhibitory concentration of sulfated polysaccharides on the enzymatic activity of sPLA2, effects on the constants Vmax and Km Finally, using the models of paw edema, swelling of skin and anti-inflammatory activity of sulfated polysaccharides In addition, we intend to evaluate the effects of these polysaccharides against myotoxicity and aggregating activity of sPLA2.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PIRES, CAMILA L.; RODRIGUES, SELMA D.; BRISTOT, DANIEL; GAETA, HENRIQUE HESSEL; TOYAMA, DANIELA DE OLIVEIRA; LOBO FARIAS, WLADIMIR RONALD; TOYAMA, MARCOS HIKARI. Sulfated polysaccharide extracted of the green algae Caulerpa racemosa increase the enzymatic activity and paw edema induced by sPLA2 from Crotalus durissus terrificus venom. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 23, n. 4, p. 635-643, . (11/14241-4, 11/06704-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PIRES, Camila Lehnhardt. Avaliação dos efeitos dos polissacarídeos sulfatados de macroalgas marinhas sobre a Fosfolipase A2 de Crotalus durissus terrificus. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.