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Effect of lyophilized bovine colostrum on the histological characteristic of the intestinal tissue of pacu (Piaractus mesopotamicus, Holmberg, 1887) and dourado (Salminus brasiliensis, Cuvier, 1816) juveniles

Grant number: 12/01855-7
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): May 01, 2012
Effective date (End): July 31, 2013
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Production
Principal Investigator:Raul Machado Neto
Grantee:Thaline Maira Pachelli da Cruz
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil


As a food of animal origin, lyophilized bovine colostrum (LBC) may prove to be an innovative alternative when added as a source of protein and bioactive peptides in fish feeding. This study will evaluate the effects of lyophilized bovine colostrum on the histology of the intestinal epithelium of juvenile neotropical endemic species fishes, such as pacu and gold. Pacu (Piaractus mesopotamicus Holmberg, 1887) and gold (Salminus brasiliensis Cuvier, 1816) fry will be distributed randomly in a 3x2x2 factorial design (12 fish per treatment in triplicate) corresponding to the experimental diets (control, 10 and 20% inclusion of LBC), the sampling dates (30 and 60 days after the beginning of the trial) and the feeding habits (omnivores and ichthyophagous). The animals will be fed twice a day until apparent satiation. On the sampling dates the intestine of seven animals will be collected and divided into medium intestine and rectum, the first subdivided into two segments, a cranial and other intermediary. These samples will be analyzed for histological features as well as partial volume of the absorptive mucosa, muscle layer thickness and quantification and differentiation of goblet cells. Additionally, the effect of IGF-I present in bovine colostrum will be evaluate on these characteristics of the intestinal epithelium.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CRUZ, Thaline Maira Pachelli da. Intestinal epithelium of pacu (Piaractus mesopotamicus, Holmberg 1887) and dourado (Salminus brasiliensis, Cuvier 1816) juveniles fed diet containing lyophilized bovine colostrum. 2013. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.

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