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Development of impedimetric immunosensor for detection of disperse Red 1 dye

Grant number: 11/15277-2
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): May 01, 2012
Effective date (End): June 30, 2014
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal Investigator:Hideko Yamanaka
Grantee:Carolina Gomes da Rocha
Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated research grant:08/10449-7 - Assessment of occurrence, toxicity/genotoxicity and degradation processes of dyes in effluents and surface water, AP.TEM


Azo dyes are among the most used by the Brazilian textile industry, representing around 20-40% of the dyes used for dying cotton, rayon, silk, nylon, wool and leather. The problem is that several investigations using microorganisms and mammalian cells have indicated that many azo dyes have genotoxic activity even at low concentrations. In this project, the development of an impedimetric immunosensor for detection of Red 1 azo dye at low levels of concentration is proposed. In this case, monoclonal antibody anti- Red 1 will be immobilized on the working electrode through protein A to provide the interaction of Fab portion with antigen present in solution. The protein A from Staphylococcus aureus will be directly fixed on the gold electrode surface modified with cystamine or through glutaraldehyde at cystamine. The optimization of experimental parameters (reagents concentrations for previous electrode modification, antibody immobilization and incubations time) for immunosensor construction will carry out by factorial design employing electrochemical impedance measurements (EIS). The device will be characterized by DRIFTS and cyclic voltammetry and then, the affinity reaction between the immobilized antibody and antigen present in solution will be monitored by EIS. This project takes part of Thematic Project entitled "Evaluation of occurrence, toxicity/genotoxicity and process for dye degradation in effluents and superficial waters", so the impedimetric immunosensor will be applied to determine the dye concentration in water samples to be investigated or investigated in others sub-projects of Thematic.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROCHA, CAROLINA G.; FERREIRA, ANTONIO A. P.; YAMANAKA, HIDEKO. Label-free impedimetric immunosensor for detection of the textile azo dye Disperse Red 1 in treated water. SENSORS AND ACTUATORS B-CHEMICAL, v. 236, p. 52-59, . (08/10449-7, 11/15277-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ROCHA, Carolina Gomes da. Desenvolvimento de imunossensor impedimétrico para detecção do corante disperso Red 1. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Química. Araraquara Araraquara.

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