| Grant number: | 11/21346-7 |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| Effective date (Start): | April 01, 2012 |
| Effective date (End): | October 31, 2014 |
| Field of knowledge: | Biological Sciences - Microbiology - Applied Microbiology |
| Principal Investigator: | Antonio Olavo Cardoso Jorge |
| Grantee: | Anna Carolina Borges Pereira da Costa |
| Host Institution: | Faculdade de Odontologia (FOSJC). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil |
Abstract The aim of this study will be analyze 9 genes of unknown function from Candida albicans, among 34 mutant strains that show altered phenotype to biofilm formation, on the cells viability, dry weight, metabolic activity and biofilme architecture, biological characteristics and testing model infections. Biofilms will be grown in 96- well plates or disks of polystyrene and evaluated at different time of development. The mutant and revertant strains will be assessed for for susceptibility to stressor, growth under nutrient limitation and filamentation tests. For models of infection, epithelial cells (TR- 146) e endothelial cells (HUVEC) will be used for analysis of adherence, invasion and damage by mutant and revertant strains. The virulence of the strains will be verified in macrophage survival assay and death of C. albicans by neutrophils. The pathogenicity will be evaluated in embryonated chicken eggs for 7 days after inoculation of the strains. According to the results obtained will be selected one or two mutant strains for specific and detailed analysis of genes/ proteins involved. The results will be analyzed by ANOVA, Tukey's test, Student's t- test, log-rank test(Mantel- Cox) and Gehan- Breslow-Wilcoxon test (P d 0.05). | |
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