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Study of the mechanisms involved in the process of differentiation into osteogenic lineage of mesenchymal stem cells from bone marrow of Wistar rats and spontaneously hypertensive rats (SHR)

Grant number: 12/01924-9
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): May 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Sandra Helena Penha de Oliveira
Grantee:Thamine Landim de Barros
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


Osteoporosis is a bone disorder that leads to reduction in bone mass and increased risk of fractures. Hypertension is a multifactorial disease, determined by genetic and environmental factors, considered a risk factor for osteoporosis. The pharmacological therapies available for the management of osteoporosis have no effect on hypertension related to osteoporosis. SHR (spontaneously hypertensive rats) are a genetic model of hypertension that showed damage bone architecture. Hyperproliferative activity and changes in insulin signaling pathway in smooth muscle cells from rat aorta SHR of 4 weeks of age has seen the influence of hypertensive genotype in cell biology of these animals, however it is unknown whether this condition can affect bone metabolism. Therefore, this study will evaluate whether the hypertensive genotype may influence the differentiation into osteogenic lineage of mesenchymal stem cells of young rats SHR (4 weeks) have not yet developed hypertension, in order to show only the influence of genotype hypertensive. To answer this question will be assessed the following parameters: gene expression of osteogenic transcription factors (Runx2 and ²-catenin) and bone matrix proteins collagen (type I collagen) and non-collagen (bone sialoprotein, osteopontin, osteocalcin); cell proliferation, total protein, alkaline phosphatase activity, formation of mineralized matrix, and modulating the activity of the signaling pathway of protein kinases activated by mitogenic agents (MAPKs - ERK 1/2, p38 and SAPK / JNK).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE BARROS, THAMINE LANDIM; BALERA BRITO, VICTOR GUSTAVO; FERREIRA DO AMARAL, CARIL CONSTANTE; CHAVES-NETO, ANTONIO HERNANDES; CAMPANELLI, ANA PAULA; PENHA OLIVEIRA, SANDRA HELENA. Osteogenic markers are reduced in bone-marrow mesenchymal cells and femoral bone of young spontaneously hypertensive rats. Life Sciences, v. 146, p. 174-183, . (12/01924-9, 11/06070-5, 11/19458-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BARROS, Thamine Landim de. Estudo dos mecanismos envolvidos no processo de diferenciação em linhagem osteogênica de células-tronco mesenquimais da medula óssea de ratos Wistar e ratos espontaneamente hipertensos (SHR). 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.

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