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Effects of biochar in mitigating heavy metals toxicity in multicontaminated soils

Grant number: 12/01799-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2012
Effective date (End): April 02, 2015
Field of knowledge:Agronomical Sciences - Agronomy - Soil Science
Principal Investigator:Cleide Aparecida de Abreu
Grantee:Aline Peregrina Puga
Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
Associated scholarship(s):13/27010-6 - Biochar-plant interactions: a study of metal absorption and root development in contaminated soils, BE.EP.DR


Heavy metals occur naturally in soils or can be increased due to human activities, mainly those associated with mining. Due to the high toxicity and accumulation in the food chain is of great importance to determine the behavior and fate of these elements in contaminated soils. Therefore, such information can help to minimize the ecotoxicological risk. With this proposal we aim to evaluate the effect of biochar (BC) on the mobility of Cd, Pb and Zn in contaminated soils at different pH values, as well as on growth and absorption of these elements, and the activity of some enzymes and structures of two plant species. In a first experiment five leaching columns will be installed with a contaminated soil by mining, with the following treatments: natural soil pH (6,0-7,0); acidified soil to reach pH H 4,0; natural soil pH with the application of BC (10 % m / m); acidified soil with application of BC (10% w/w); lime application to soil to raise pH H 8,0. The BC will be produced and characterized in a previous study from sugar cane straw. The leachate will be analyzed for Cd, Pb and Zn concentrations , water soluble carbon, electrical conductivity and pH. After leachings the soil will be sequentially extracted to study the distribution of these elements in different compartiments. In another experiment the effect of BC addition on the absorption and accumulation of Cd, Pb and Zn in ryegrass and tobacco will be evaluated. Besides, we will evaluate the dry mass yield, the activities of catalase and guaiacol peroxidase, lipid peroxidation, and the alterations in the structure and ultrastructure by using scanning electron microscope. BC will be applied to the soil at 0% (control), 2%, 5% and 10% (m / m) rates, with four replicates. The soils of this experiment will be extracted by DTPA pH 7,3 and 0,01 M CaCl2 to determine the available contents of heavy metals, which will be tested for correlation with the content in shoots. The obtained results will be analyzed by ANOVA, as significant (p <0,05) means will be compared by Tukey test (p <0,05), polynomial regression and correlation analysis to diferentiate the treatments.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PUGA, A. P.; ABREU, C. A.; MELO, L. C. A.; PAZ-FERREIRO, J.; BEESLEY, L.. Cadmium, lead, and zinc mobility and plant uptake in a mine soil amended with sugarcane straw biochar. Environmental Science and Pollution Research, v. 22, n. 22, p. 17606-17614, . (12/01799-0, 14/04454-9)
PUGA, A. P.; ABREU, C. A.; MELO, L. C. A.; BEESLEY, L.. Biochar application to a contaminated soil reduces the availability and plant uptake of zinc, lead and cadmium. Journal of Environmental Management, v. 159, p. 86-93, . (12/01799-0, 14/04454-9)

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