Ischemic acute kidney injury (AKI) triggers a cascade of events involving inflammatory response and generation of reactive oxygen species that result in endothelial and tubular injury. Moreover, the active mechanism of hypoxia induces adaptation and cytoprotection as the enzyme heme oxygenase-1 (HO-1). The sildenafil citrate confers anti-inflammatory properties and vascular protection. The objective of this study is to evaluate the effect the sildenafil citrate in the LRA in ischemic rats according to the severity of kidney injury. Material and Methods: Wistar rats, adults and males (250 to 300g), divided into the following groups: SHAM (surgery simulation); Sildenafil (sildenafil 0,25 mg/kg, v.o., 1 day); Ischemia 30 min (renal clamping for 30 min); Ischemia 45 min (clamping for 45 min); Ischemia 30 min + sildenafil; Ischemia 45 min + sildenafil. Renal function, oxidative profile, release of nitric oxide (NO), HO-1 and inducible nitric oxide synthase (iNOS) gene expression and histology of renal tissue will be evaluated.
News published in Agência FAPESP Newsletter about the scholarship: