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Research of encoding genes of staphylococcal enterotoxins and vancomycin resistance gene vanA: detection of genic expression in Staphylococcus coagulase positive and negative in bovine mastitis

Grant number: 11/21142-2
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2012
Effective date (End): February 28, 2015
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:Helio Langoni
Grantee:Felipe de Freitas Guimarães
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


The concern of the genus Staphylococcus and importance have increased substantially, in animals and mainly in humans. Currently in addition to S. aureus, other coagulase-positive staphylococci (CPS) and coagulase-negative staphylococci (SCN) considered of minor importance, are attracting the interest of researchers because of the risk to public health represented by these micro-organisms in the etiology of mastitis in relation to antimicrobial resistance production capacity and heat-stable enterotoxin. The participation of these pathogens in bovine mastitis by milk and broadcasting justifies the need to investigate the toxigenic capacity and antimicrobial resistance. The objectives of this project: identify the main species, determine the potential risk to public health represented by the toxigenic capacity of strains and vancomycin resistance by detection of the vanA gene. It will be studied 150 strains of SCP and SCN 150 strains isolated from cases of bovine mastitis from different properties of the State of Sao Paulo. From the isolates will be analyzed for the presence of genes for production of enterotoxins by Polymerase Chain Reaction (PCR). Gene expression will be evaluated by Real-Time PCR (RTQ-PCR). The isolates will be subjected to sensitivity profile correlated with the detection of the vanA gene by PCR. The relevance of this project is based on the need to contribute to assessing the risk of mastitis by SCP and SCN in the food security of consumers of milk. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FELIPE FREITAS GUIMARÃES; SAMEA FERNANDES JOAQUIM; MARCELA PINHO MANZI; RODRIGO COSTA DA SILVA; ARIANE CRISTINA MENDES DE OLIVEIRA BRUDER-NASCIMENTO; ELIZABETH OLIVEIRA COSTA; HÉLIO LANGONI. Comparação da identificação fenotípica e genotípica de espécies do gênero Staphylococcus isoladas de mastite bovina. Pesquisa Veterinária Brasileira, v. 36, n. 12, p. 1160-1164, . (11/21323-7, 11/21142-2)
GUIMARAES, F. F.; MANZI, M. P.; JOAQUIM, S. F.; RICHINI-PEREIRA, V. B.; LANGONI, H.. Short communication: Outbreak of methicillin-resistant Staphylococcus aureus (MRSA)-associated mastitis in a closed dairy herd. JOURNAL OF DAIRY SCIENCE, v. 100, n. 1, p. 726-730, . (08/11614-1, 11/21323-7, 08/08780-7, 11/21142-2)
GUIMARAES, FELIPE DE FREITAS; NOBREGA, DIEGO BONIN; RICHINI-PEREIRA, VIRGINIA BODELAO; MARSON, PAMELA MERLO; DE FIGUEIREDO PANTOJA, JOSE CARLOS; LANGONI, HELIO. Enterotoxin genes in coagulase-negative and coagulase-positive staphylococci isolated from bovine milk. JOURNAL OF DAIRY SCIENCE, v. 96, n. 5, p. 2866-2872, . (08/11614-1, 11/21142-2, 08/08780-7)
GUIMARAES, FELIPE FREITAS; JOAQUIM, SAMEA FERNANDES; MANZI, MARCELA PINHO; DA SILVA, RODRIGO COSTA; MENDES DE OLIVEIRA BRUDER-NASCIMENTO, ARIANE CRISTINA; COSTA, ELIZABETH OLIVEIRA; LANGONI, HELIO. Comparison phenotypic and genotypic identification of Staphylococcus species isolated from bovine mastitis(1). Pesquisa Veterinária Brasileira, v. 36, n. 12, p. 5-pg., . (11/21142-2, 11/21323-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GUIMARÃES, Felipe de Freitas. Detection of enterotoxins enconding genes, phenotypic and genotypic antimicrobial resistence, mecA and vanA and genic expression in bovine mastite cases by staphylococcus coagulase positive and negative isolates. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.

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