Advanced search
Start date

"Histatin-5 action, an antimicrobial peptide, on planktonic cells and biofilm development of Candida. Albicans."

Grant number: 11/23543-4
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2012
Effective date (End): March 31, 2015
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Eunice Teresinha Giampaolo
Grantee:Eduardo Buozi Moffa
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated scholarship(s):13/15412-2 - Analysis of Histatin 5 salivary complexes by using mass spectrometry, BE.EP.DR


The widespread use of topical and systemic antifungal agents as conventional treatment for oral candidosis has resulted in the development of resistance in C. albicans. Thus, it is necessary to develop alternative therapies for the treatment of oral candidosis. Histatin 5 is a human basic salivary peptide with strong fungicidal properties in vitro. This peptide is extremely rich in one or two specific amino acids, which are positively charged at physiological pH and inhibit biofilm formation. Thus, the aim of this study is to i) synthesize, purify and characterize Histatins-5, ii) determine of the minimum inhibitory concentration (MIC) of the agent on planktonic cells and biofilms of Candida albicans by XTT assay and by counting viable colonies, iii) determine the concentration and establish the appropriate immersion times required to promote the inactivation of micro-organisms using similar tests described above; iv) evaluate in vitro the cytotoxicity of this peptide using tests mitochondrial activity (MTT) and electron microscopy (SEM). Theses analysis will allow us the development of a protocol for prevention and treatment of candidiasis in denture users.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MOFFA, EDUARDO B.; MUSSI, MARIA C. M.; XIAO, YIZHI; GARRIDO, SAULO S.; MACHADO, MARIA A. A. M.; GIAMPAOLO, EUNICE T.; SIQUEIRA, WALTER L.. Histatin 5 inhibits adhesion of C. albicans to Reconstructed Human Oral Epithelium. FRONTIERS IN MICROBIOLOGY, v. 6, . (11/23540-5, 11/23543-4, 13/15412-2)
MOFFA, EDUARDO B.; MACHADO, MARIA A. A. M.; MUSSI, MARIA C. M.; XIAO, YIZHI; GARRIDO, SAULO S.; GIAMPAOLO, EUNICE T.; SIQUEIRA, WALTER L.. In Vitro Identification of Histatin 5 Salivary Complexes. PLoS One, v. 10, n. 11, . (12/01528-6, 11/23540-5, 11/23543-4, 13/15412-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MOFFA, Eduardo Buozi. Ação da histatina 5, uma proteína antimicrobiana, no desenvolvimento de biofilmes de Candida Albicans. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.

Please report errors in scientific publications list by writing to: