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Functional analysis of cell signaling involvement in cancer stem cells obtained from oral squamous cell carcinoma cell lines

Grant number: 11/21395-8
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): April 01, 2012
Effective date (End): May 31, 2015
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Fabio Daumas Nunes
Grantee:Maria Fernanda Setúbal Destro Rodrigues
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:10/51168-0 - Environmental, clinical, histopathological and molecular factors associated with development and prognosis of head and neck squamous cell carcinomas, AP.TEM
Associated scholarship(s):14/12658-3 - Effects of cetuximab treatment in the behaviour of cancer stem cell in oral squamous cell carcinoma, BE.EP.PD


Oral squamous cell carcinoma (OSCC) is the most prevalent neoplasia of oral cavity. The comprehension of this neoplasia is limited regarding its origin and which cells are involved in tumor development and progression, difficulting to establish an effective treatment and preventive strategies. The identification of neoplastic cells subpopulations on oral squamous cell carcinoma fundamental for tumoral expansion and metastasis, is essential to improve treatment and increase survival rates of patients OSCC. Recently, it was reported that head and neck squamous cell carcinomas presents a subpopulation of tumoral initiators cells that apparently correspond to malignant stem cells, also responsible for tumor growth. This knowledge raised the perspective of new therapeutic approaches, and a better understanding of tumor development mechanisms. Despite several advances on stem cells research, there are few studies focused on the analysis of oral squamous cell carcinoma stem cells. The main purpose of the present study is the isolation and characterization of oral squamous cell carcinoma stem cells from established cell lines using specifics cell markers. Additionally, the gene expression profile of these cells will be analyzed to identify important cell signaling molecules for the regulation of OSCC stem cells, and also, their functional hole in controlling essentials cellular processes to the malignant transformation. The protein expression of these genes will be evaluated in OSCC and non-neoplastic oral tissue samples and subsequently related to clinicopathological features and overall survival.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SETUBAL DESTRO RODRIGUES, MARIA FERNANDA; MIGUITA, LUCYENE; DE ANDRADE, NATHALIA PAIVA; HEGUEDUSCH, DANIELE; RODINI, CAMILA OLIVEIRA; MOYSES, RAQUEL AJUB; TOPORCOV, TATIANA NATASHA; GAMA, RICARDO RIBEIRO; TAJARA, ELOIZA ELENA; NUNES, FABIO DAUMAS. GLI3 knockdown decreases stemness, cell proliferation and invasion in oral squamous cell carcinoma. International Journal of Oncology, v. 53, n. 6, p. 2458-2472, . (12/00786-1, 11/21395-8)
DE ANDRADE, NATHALIA PAIVA; SETUBAL DESTRO RODRIGUES, MARIA FERNANDA; RODINI, CAMILA OLIVEIRA; NUNES, FABIO DAUMAS. Cancer stem cell, cytokeratins and epithelial to mesenchymal transition markers expression in oral squamous cell carcinoma derived from ortothopic xenoimplantation of CD44(high) cells. PATHOLOGY RESEARCH AND PRACTICE, v. 213, n. 3, p. 235-244, . (12/00786-1, 12/20622-3, 11/21395-8)
SETUBAL DESTRO RODRIGUES, MARIA FERNANDA; TOBOUTI, PRISCILA LIE; MOLON, ANGELA CRISTINA; SEDASSARI, BRUNO TAVARES; NUNES, FABIO DAUMAS; PINTO JUNIOR, DECIO DOS SANTOS; ORSINI MACHADO DE SOUSA, SUZANA CANTANHEDE. Histopathological findings and immunohistochemical expression of the stem cell markers CD44, ALDH1, Bmi-1, and Nanog in oral solitary fibrous tumors. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 131, n. 4, p. 444-451, . (11/21395-8, 12/00786-1)

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