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Influence of different irrigating solution associated to the photodynamic therapy: in vitro and in vivo study

Grant number: 11/23287-8
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): March 01, 2012
Effective date (End): May 07, 2014
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:João Eduardo Gomes Filho
Grantee:Gustavo Sivieri de Araújo
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


Recently, it has been showed strong evidence of the photodynamic therapy (PDT) success in microbial reduction. PDT is based on appropriate physical, chemical and biological effects, that occur after administration of a photosensitizing agent (PS) activated by visible light of specific wavelength, with intent to destroy the target cell. The aim of this study is to evaluate the behavior of PDT in comparison with some irrigating solutions, as well as the association between the irrigating solutions and PDT using different methodologies: 1-antimicrobial effects in vitro by direct contact test against strains of Enterococcus faecalis and Porphyromonas gingivalis. The results will be evaluated with ANOVA, Tukey and Bonferroni's tests (p <0.05), 2-cytotoxicity in L-929 fibroblasts by the MTT colorimetric method, 3-Production of cytokines (IL-1² and IL-6) by fibroblasts using enzyme immunoassay (ELISA). The results will be statistically analyzed by ANOVA with Bonferroni's correction (p <0.05), 4- tissue response to polyethylene tubes implant in the subcutaneous tissue of rats. The results will be statistically analyzed by Kruskal-Wallis test (p <0.05), 5-Production of cytokines (IL-1² and IL-6) by the tissue, analyzed by ELISA. The results will be statistically analyzed by ANOVA with Bonferroni's correction (p <0.05). 6 - Analysis of periapical healing in the treatment of dogs' teeth with chronic periapical lesion in one session. The results will be analyzed by Kruskal Wallis statistical test (p <0.05), 7 - Quantification of the microbiota present in the root canal system before and after biomechanical preparation in one appointment in dogs' teeth with chronic periapical lesion, using the RT-PCR in real time. It will be applied ANOVA and Tukey's test (p <0.05).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SIVIERI-ARAUJO, GUSTAVO; DE AZEVEDO QUEIROZ, INDIA OLINTA; DAL FABBRO, RENAN; ESTEVES, FERNANDA; ANGELO CINTRA, LUCIANO TAVARES; TOBIAS DUARTE, PAULO CARVALHO; BAGNATO, VANDERLEI SALVADOR; PENHA OLIVEIRA, SANDRA HELENA; GOMES-FILHO, JOAO EDUARDO. Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy. Photodiagnosis and Photodynamic Therapy, v. 18, p. 315-318, . (12/12696-7, 11/23287-8, 12/06785-7)
GOMES-FILHO, JOAO EDUARDO; SIVIERI-ARAUJO, GUSTAVO; SIPERT, CARLA RENATA; DA SILVA SANTOS, LUDMILLA MOTA; DE AZEVEDO QUEIROZ, INDIA OLINTA; MARTINS, CHRISTINE MEN; DO CARMO MAIA, NAYARA KIVILLA; ANGELO CINTRA, LUCIANO TAVARES; DEZAN-JUNIOR, ELOI; BAGNATO, VANDERLEI SALVADOR; et al. Evaluation of photodynamic therapy on fibroblast viability and cytokine production. Photodiagnosis and Photodynamic Therapy, v. 13, p. 97-100, . (12/06785-7, 11/23287-8, 12/12695-0)

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