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Immunomodulation of experimental autoimmune encephalomyelitis by salivary gland extract of Aedes aegypti

Grant number: 11/22669-4
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2012
Effective date (End): July 31, 2014
Field of knowledge:Biological Sciences - Immunology - Cellular Immunology
Principal Investigator:Anderson de Sá Nunes
Grantee:Anderson Daniel Ramos
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:09/09892-6 - Functional immunome of Aedes aegypti saliva, AP.JP


During a successful blood feeding of A. aegypti female mosquito, various biologically active components of saliva interact with the host and alter a number of hematological and immunological factors. Together, these modifications can lead to profound changes in the course of some infectious and autoimmune diseases. In this sense, several immunomodulatory activities have been described in the saliva of bloodsucking insects in recent years. Thus, the aim of this proposal is to analyze the modulation of experimental autoimmune encephalomyelitis (EAE), a model for studying of multiple sclerosis, by the salivary components of the mosquito A. aegypti in C57BL/6 mice. A. aegypti salivary gland extract (SGE) will be produced from the salivary glands of females with 5-7 days after emerging. This material will be sonicated, centrifuged, sterilized, the protein content will be measured and stored at -80 °C until use. To study the in vivo activity of SGE on EAE (induced by immunization with MOG p35-55), we will use two distinct approaches. In the preventive approach, a group of immunized animals will receive a daily treatment with SGE starting at the day two of immunization, administered intravenously. In therapeutic approach, animals immunized will be treated with SGE in the same dose and route at the beginning of the first signs of the disease, determined by a well known clinical score. The disease progression will be monitored for 30 days and compared with a group of animals given only vehicle (PBS) in the same treatment regimen and route. In both cases, some animals will be euthanized for histopathological analysis of the central nervous system (CNS) as well as parameters related to host immunity, such as cytokine production by cells from lymph nodes and frequencies of lymphocyte subpopulations in these organs. We also will perform a fractionation of the SGE in order to identify one or more fractions in an attempt to characterize biologically active molecules in this model. We hope to demonstrate the activity of A. aegypti SGE in a model of autoimmunity, while we characterize new potential immunomodulatory molecules in mosquito saliva. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MACIEL, CERES; FUJITA, ANDRE; GUERONI, DANIELE I.; RAMOS, ANDERSON D.; CAPURRO, MARGARETH L.; SA-NUNES, ANDERSON. Evans Blue as a Simple Method to Discriminate Mosquitoes' Feeding Choice on Small Laboratory Animals. PLoS One, v. 9, n. 10, . (09/53637-0, 10/18216-1, 09/09892-6, 11/22669-4)
BARROS, MICHELE S.; GOMES, ELIANE; GUERONI, DANIELE I.; RAMOS, ANDERSON D.; MIROTTI, LUCIANA; FLORSHEIM, ESTHER; BIZZARRO, BRUNA; LINO, CIRO N. R.; MACIEL, CERES; LINO-DOS-SANTOS-FRANCO, ADRIANA; et al. Exposure to iBites Induces a Mixed-Type Allergic Response following Salivary Antigens Challenge in Mice. PLoS One, v. 11, n. 5, . (11/15569-3, 10/18216-1, 09/09892-6, 09/07208-0, 09/12247-5, 11/22669-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
RAMOS, Anderson Daniel. Immunomodulation of experimental autoimmune encephalomyelitis by salivary gland extract of Aedes aegypti.. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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