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Funcionalization of silver nanoparticles with beta-lactam antibiotics: an alternative to bacterial resistance

Grant number: 11/15937-2
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): January 31, 2014
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
Principal Investigator:Mateus Borba Cardoso
Grantee:Jessica Fernanda Affonso de Oliveira
Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil

Abstract

The project here described aims to synthesize silver nanoparticles (AgNP) encapsulated into mesoporous silica (MS) for further functionalization with beta-lactams antibiotics. Three different beta-lactams agents will be tested: penicillin G, amoxicillin and ampicillin. Since AgNPs are effective antibacterial agents, the main propose of this work is to obtain a dual bacterial inhibition system, containing AgNPs and beta-lactams agents. Moreover, this work will focus on: (1) analyzing the possible synergism of using AgNPs combined with beta-lactams antibiotics against non-resistant bacteria and (2) investigating a possible bactericidal action of the synthesized systems against beta-lactams resistant bacterias. The structures formed will be characterized by ultraviolet visible spectroscopy (UV-Vis), small angle X-ray scattering (SAXS), transmission electron microscopy (TEM), X-ray diffraction (XRD) and thermo-gravimetric analysis (TGA). For those structures containing mesoporous silica, nitrogen adsorption/desorption isotherms will be used in determining the size and organization of the pores. The evolution of the nanoparticles functionalization will be investigated by Fourier transform infrared spectroscopy (FT-IR) and zeta potential. Biological activity experiments will be conducted with Escherichia coli (gram negative), Staphylococcus aureus, Staphylococcus epidermidis and Micrococcus lysodeikticus (gram positive), using the disk diffusion method. Finally, the minimum inhibitory concentration for each system will be determined by nanoparticles incubation test in tubes containing the bacteria.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AFFONSO DE OLIVEIRA, JESSICA FERNANDA; SAITO, ANGELA; BIDO, ARIADNE TUCKMANTEL; KOBARG, JOERG; STASSEN, HUBERT KARL; CARDOSO, MATEUS BORBA. Defeating Bacterial Resistance and Preventing Mammalian Cells Toxicity Through Rational Design of Antibiotic-Functionalized Nanoparticles. SCIENTIFIC REPORTS, v. 7, . (14/22322-2, 11/15937-2, 15/25406-5)
AFFONSO DE OLIVEIRA, JESSICA FERNANDA; CARDOSO, MATEUS BORBA. Partial Aggregation of Silver Nanoparticles Induced by Capping and Reducing Agents Competition. Langmuir, v. 30, n. 17, p. 4879-4886, . (11/21954-7, 11/15937-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
OLIVEIRA, Jessica Fernanda Affonso de. Functionaliztion of silver nanoparticles with 'beta'-lactam antibiotics: an alternative to bacterial resistance. 2014. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.

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