Histomorphometric and gene expression evaluation of bacterial celulose composites with addition of growth factor regulatory peptide in the bone regeneration

Abstract

In recent years, biotechnology has greatly advanced in the development of biomaterials such as resorbable or nonresorbable membranes for the regeneration of bone or skin lesions. However, the ideal would be developing materials which also induced osteogenesis, were biocompatible, readily available in the market and low cost. The Chemistry Institute (IQ) of the São Paulo State University (UNESP) Araraquara Campus, developed from bacterial cellulose (BC) composites that help in bone regeneration with hydroxyapatite (HA). A few years ago an important partnership was established between IQ and teachers from Araraquara School of Dentistry (FOAr - UNESP). In addition to the complete chemical characterization of the mentioned composites, very promising results were obtained from these biochemical and histological assessment when used for bone regeneration after non-critical defect in the femur of rats. One of the biomaterials with improved osteogenic effect was BC-HA composite with adsorbed pentapeptide OGP [10-14]. This study aims to evaluate the potential of CB-based biomaterials in bone regeneration after critical defect of mice's calvaria. 105 animals will be used to assess membrane BC (group I) and HA-BC OGP [10-14] composite (group II), comparing them to the cloth (group III), using the methods of histomorphometry and gene expression analysis. The results will be submitted to statistical parametric or nonparametric analysis, depending on the adherence of the data to the normality curve. All tests will be performed at the significance level of 5% using the SAS and BioEstat v.5.0 programs. This study is expected to know which biomaterials have better performance in bone regeneration, and the participation of the gene expression in this process. The expansion of knowledge in the biological potential of biomaterials developed in IQ will contribute to their further use in the medical device industry.