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Identification of Fusarium species from clinical specimens from Campinas State University Hospital through the techniques of DNA microarray and loop-mediated isothermal amplification (LAMP).

Grant number: 11/16205-5
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Plinio Trabasso
Grantee:Marcela de Souza
Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


This study aims to identify filamentous fungi through "DNA microarray" method. The microorganisms of bloodstream fungal infection will be isolated from patients with onco hematologic diseases, mainly acute leukemia or lymphoma, cared for at hematology ward of the Campinas State University Hospital (HC-UNICAMP). The strains of filamentous fungi will be initially isolated from routine exams of patients, performed by the Division of Mycology of Clinical Pathology Laboratory. The isolates will be identified using a combination of macroscopic and micromorphological features, and will be subcultured and analyzed in platform of oligonucleotide sequences (DNA microarray). The platform will be developed at the University of Campinas, based on oligonucleotide sequences previously defined and plotted on plastic slides for the more frequent molds in this population, namely Fusarium solani complex, Fusarium oxysporum, Fusarium moniliforme, Fusarium sacchari ,F usarium glicynes, delphinoides Fusarium, Fusarium napiforme, sickle cell Fusarium, Aspergillus, Rhizopus, Rhizomucor, Mucor, Scedosporium, Exophiala, Absidia, Cladosporium.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE SOUZA, MARCELA; MATSUZAWA, TETSUHIRO; SAKAI, KANAE; MURAOSA, YASUNORI; LYRA, LUZIA; BUSSO-LOPES, ARIANE FIDELIS; SHAFFERMAN LEVIN, ANNA SARA; SCHREIBER, ANGELICA ZANINELLI; MIKAMI, YUZURU; GONOI, TOHORU; et al. Comparison of DNA Microarray, Loop-Mediated Isothermal Amplification (LAMP) and Real-Time PCR with DNA Sequencing for Identification of Fusarium spp. Obtained from Patients with Hematologic Malignancies. Mycopathologia, v. 182, n. 7-8, p. 625-632, . (11/16205-5)
DE SOUZA, MARCELA; MATSUZAWA, TETSUHIRO; LYRA, LUZIA; BUSSO-LOPES, ARIANE FIDELIS; GONOI, TOHRU; SCHREIBER, ANGELICA ZANINELE; KAMEI, KATSUHIKO; MORETTI, MARIA LUIZA; TRABASSO, PLINIO. Fusarium napiforme systemic infection: case report with molecular characterization and antifungal susceptibility tests. SPRINGERPLUS, v. 3, . (11/16205-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SOUZA, Marcela de. Comparison of molecular techiniques for identification of fusarium species from clinical samples. 2014. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.

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