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Effects of resistance exercise on trophic and atrophic patways in skeletal muscle of rats with paradoxal sleep deprivation

Grant number: 11/15962-7
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): August 31, 2013
Field of knowledge:Health Sciences - Physical Education
Principal Investigator:Marco Túlio de Mello
Grantee:Marcos Mônico Neto
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil


Sleep is an important factor in the process of muscle recovery. Changes caused by sleep deprivation and sleep restriction, cause damage in specific hormonal axes, such as hypothalamic-pituitary-adrenal and hypothalamic-pituitary-gonadal axis. The increase on the secretion of corticosterone and decreases testosterone, growth hormone (GH) and insulin-like growth factor-1 (IGF-1) levels, creates a catabolic environment and promotes the process of muscle atrophy. On the other hand, resistance exercise promotes important physiological adaptations, with emphasis on the metabolic, hormonal and intracellular respond by increasing strength, cross-sectional area and maintenance / synthesis of contractile proteins. The aim of this project is evaluate the effects of resistance exercise in the way trophic and atrophic skeletal muscle of rats subjected to deprivation of paradoxical sleep for 96 hours. Will be used male rats, subjected to a protocol of high intensity resistance training for 8 weeks followed by a protocol of paradoxical sleep deprivation for 96 hours. After the animals are euthanized and gastrocnemius muscle blood will be taken for biochemical, histological and blood. To evaluate the anabolic and catabolic hormonal responses will be measured levels of testosterone, IGF-1 and corticosterone. Histology of the gastrocnemius muscle is made with hematoxylin and eosin (HE) and ATPase histomorphometry to evaluate the different types of muscle fibers. Biochemical analysis will be performed by Western blotting of proteins key pathways of synthesis and protein degradation, and the AKT, mTOR, p70S6K1, FOXO, MuRF1, and myostatin and their active forms.

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MONICO-NETO, MARCOS; MOREIRA ANTUNES, HANNA KAREN; LEE, KIL SUN; PHILLIPS, STUART M.; DE CAMPOS GIAMPA, SARA QUAGLIA; SOUZA, HELTON DE SA; DATTILO, MURILO; MEDEIROS, ALESSANDRA; DE MORAES, WILSON MAX; TUFIK, SERGIO; et al. Resistance training minimizes catabolic effects induced by sleep deprivation in rats. APPLIED PHYSIOLOGY NUTRITION AND METABOLISM, v. 40, n. 11, p. 1143-1150, . (13/00152-5, 11/15962-7, 98/14303-3)
MONICO-NETO, MARCOS; DE CAMPOS GIAMPA, SARA QUAGLIA; LEE, KIL SUN; DE MELO, CAMILA MARIA; SOUZA, HELTON DE SA; DATTILO, MURILO; MINALI, PAULO ALEXANDRE; SANTOS PRADO, PEDRO HENRIQUE; TUFIK, SERGIO; DE MELLO, MARCO TULIO; et al. Negative Energy Balance Induced by Paradoxical Sleep Deprivation Causes Multicompartmental Changes in Adipose Tissue and Skeletal Muscle. INTERNATIONAL JOURNAL OF ENDOCRINOLOGY, . (13/00152-5, 11/15962-7, 98/14303-3)
PEREIRA, GABRIELA CRUZ; NETO, MARCOS MONICO; MOREIRA ANTUNES, HANNA KAREN; LEE, KIL SUN; MELLO DA LUZ, MARCIO HENRIQUE. Anesthesia can alter the levels of corticosterone and the phosphorylation of signaling molecules. BMC RESEARCH NOTES, v. 14, n. 1, . (17/10404-2, 13/00152-5, 11/15962-7, 16/04297-6)
SARA QUAGLIA DE CAMPOS GIAMPÁ; MARCOS MÔNICO-NETO; HELTON DE SÁ SOUZA; MARCO TÚLIO DE MELLO; SERGIO TUFIK; LESLIE ANDREWS PORTES; ANDREY JORGE SERRA; PAULO JOSÉ FERREIRA TUCCI; HANNA KAREN MOREIRA ANTUNES. Efeito do Treinamento Resistido na Contratilidade Miocárdica In Vitro após a Privação de Sono. International Journal of Cardiovascular Sciences, v. 30, n. 1, p. 20-31, . (13/00152-5, 11/15962-7)
MONICO-NETO, M.; ANTUNES, H. K. M.; DATTILO, M.; MEDEIROS, A.; SOUZA, H. S.; LEE, K. S.; DE MELO, C. M.; TUFIK, S.; DE MELLO, M. T.. Resistance exercise: A non-pharmacological strategy to minimize or reverse sleep deprivation-induced muscle atrophy. Medical Hypotheses, v. 80, n. 6, p. 701-705, . (11/15962-7, 98/14303-3)
MONICO-NETO, MARCOS; DATTILO, MURILO; RIBEIRO, DANIEL ARAKI; LEE, KIL SUN; DE MELLO, MARCO TULIO; TUFIK, SERGIO; MOREIRA ANTUNES, HANNA KAREN. REM sleep deprivation impairs muscle regeneration in rats. GROWTH FACTORS, v. 35, n. 1, p. 12-18, . (13/00152-5, 09/11056-1, 11/15962-7, 98/14303-3)

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