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Encephalic inflammatory changes and the correlation with lymphocytes and dendritic cells population in lymphonodes of the head of dogs with visceral leishmaniosis.

Grant number: 11/15523-3
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): August 31, 2013
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal Investigator:Gisele Fabrino Machado
Grantee:José Eduardo dos Santos Silva
Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Associated scholarship(s):12/25249-9 - Evaluation of gene expression of toll-like receptors and NOD-like receptors in polymorphonuclear and mononuclear cells in the peripheral blood of dogs with visceral leishmaniasis, BE.EP.MS


The infection caused by Leishmania spp. results in a disease that presents a variety of clinical manifestations both in humans and dogs. These manifestations can range from observation of asymptomatic infected dogs to disorders that can result in the death of the host. Chronically infected dogs may show neurological symptoms, however there are few reports that characterize the lesions and elucidate the pathogenesis of visceral leishmaniasis within the central nervous system. In previous projects, it was possible to verify the presence of inflammatory infiltrate in the brain of dogs with leishmaniasis, mainly composed of CD3+ T lymphocytes and an increase in the intensity of labeling of GFAP+ astrocytes and RCA-1+ microglia. With the aim to evaluate the mechanisms involved in the pathogenesis of the brain lesions in these dogs, we propose to characterize the T lymphocytes subsets in the retropharyngeal and submandibular lymph nodes, which are considered to be involved in the brain immune surveillance. T cells will be evaluated by flow cytometry and the phenotypes evaluated will be CD3+, CD4+, CD8+, TCR ±² and TCR ³´. Dendritic cells will be also characterized using MHC-II and CD11c. Histological sections of the brain of the same dogs will also be obtained for evaluation and characterization of brain lesions and detection of T lymphocytes.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, José Eduardo dos Santos. Avaliação das lesões encefálicas e fenotipagem de linfócitos T e células dentríticas em linfócitos de cães com leishmaniose visceral. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária. Araçatuba Araçatuba.

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