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Establishment of lung maturity in lambs born at term and preterm by cytological analysis, Clements test and lamellar bodies count in amniotic fluid.

Grant number: 11/14933-3
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): May 31, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:Francisco Leydson Formiga Feitosa
Grantee:Natália Cristina de Souza
Home Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


Clinical and laboratory profiles of lambs born at term and preterm lambs will be evaluated. Twenty four lambs will be used during the experiment: eight lambs born at term at 145 days, eight preterm lambs born at approximately 138 days of life, and eight preterm lambs whose mothers received dexamethasone, two days before cesarean section. The lambs will be submitted to physical evaluation over 48 hours after birth, according to vitality, color of mucosa , rectal temperature, heart rate and respiratory characteristics (frequency and breathing pattern). The amniotic fluid will be colected from all sheeps, by aspiration, and samples will be evaluated by cytology, Clements test and lamellar bodY count. Blood samples will be collected from lambs at birth, 15 minutes, 60 minutes, and at six, 12, 24 and 48 hours in order to determine the globular volume, hemoglobin, white blood cells count and protein profile (total protein and electrophoretic fractions), as well haemogasometric variables and levels of cortisol, glucose and serum lactate. The pulmonary radiological pattern of lambs born at term and preterm lambs will be identified immediately after birth, at 24 and 48 hours of life.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NATÁLIA CRISTINA DE SOUZA; FERNANDA BOVINO; LARISSA GABRIELLA AVILLA; MAURÍCIO DESCHK; JEFFERSON F. ALCINDO; MARIA FERNANDA C.B. FINK; LUIZ CLÁUDIO N. MENDES; FRANCISCO LEYDSON F. FEITOSA. Avaliação da maturidade pulmonar fetal utilizando a análise de líquido amniótico de ovelhas que deram à luz prematuramente e a termo. Pesquisa Veterinária Brasileira, v. 40, n. 12, p. 1039-1047, Dez. 2020. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SOUZA, Natália Cristina de. Avaliação da maturidade pulmonar de cordeiros nascidos a termo e prematuros pela análise citológica, Test de Clements e contagem de corpos lamelares do líquido amniótico. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária. Araçatuba Araçatuba.

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