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Detection of virulence genes and identification of the profile of isolated clonal Staphylococcus aureus colonizing the nasopharynx obtained in a population-based study in Botucatu-SP

Grant number: 11/13818-6
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): October 31, 2012
Field of knowledge:Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
Principal Investigator:Maria de Lourdes Ribeiro de Souza da Cunha
Grantee:Lígia Maria Abraão
Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


Recent findings report the increasing incidence and severity of infections caused by S. aureus. This fact have been worsened by the wide spread of methicillin resistant (Methicillin Resistant S. aureus MRSA) isolated in nosocomial environment, and its recent introduction in the community. The main reason for the persistence and dissemination of S. aureus among humans populations is the nasal colonization. Therefore surveys studies in the context of nasal colonization are important to estimate the S. aureus burden in general and also concerning MRSA in the community. The awareness about bacterial and host relationship together to the virulence factors involved are necessary in order to provide the best option to control the infections that offer risk to health of the population. The aims of this work are to study the distribution of the MRSA clones among the population in the urban area in Botucatu, SP, to identify the acquiring risk factors and the prevalence of the virulence factors in MRSA isolates from nasopharynx in healthy individuals of the community. Moreover, will be selected 672 individuals over than one year-old stratified by place of residence, gender and age. It will be collected nasal discharge with a swab and then cultured in culture medium. At the same time, demographic and clinical data of the included individuals will be collected. S. aureus samples will be submitted to the susceptibility test to oxacillin (phenotypic and genotypically) and if resistant it will follow the SCCmec characterization. The clonal characterization will be performed in order to characterize the endemic clusters in the population and the detection of virulence genes by the polymerase chain reaction (PCR). Statistical analysis will identify the risk factors for the S. aureus carriage in general and the MRSA carriage, associated to the virulence factors from the samples isolated from healthy individuals in the community. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ABRAÃO, Lígia Maria. Detecção dos genes de virulência e identificação do perfil clonal de isolados de Staphylococcus aureus colonizantes de nasofaringe otbtidos em estudo de base populacional. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu Botucatu.

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