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Adjuvant effect of Bacillus subtilis spores co-administered with DNA vaccines

Grant number: 11/13805-1
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): September 30, 2013
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Luis Carlos de Souza Ferreira
Grantee:Luana Raposo de Melo Moraes Aps
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Bacillus subtilis spores have a record of use as probiotics and, presently, represent an alternative vaccine delivery approach. In additions, recent studies have demonstrated that B. subtilis spores are endowed with strong adjuvant effects for vaccines based on purified proteins. The aim of the present project is to evaluate the role of B. subtilis spores as adjuvants applied to DNA vaccines delivered via different administration routes, with emphasis to the intradermal (i.d.) route. As an experimental model we employed a DNA vaccine (pgDE7) encoding the E7 protein of the type 16 human papilloma virus (HPV-16) genetically fused to the type 1 human herpes virus (HSV-1) gD protein. The spore adjuvant effects will be monitored by the induction of antigen-specific immune responses and by the anti-tumor protection effects in C57BL/6 mice previously transplanted with transformed cells (TC1 cells) carrying oncogenes encoding the HPV-16 E6 and E7 proteins. Our results have indicated that bacterial spores exert strong adjuvant effects on the immune responses induced by DNA vaccines and may represent, therefore, an useful alternative for increasing the performance of this kind of vaccine. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
APS, LUANA R. M. M.; DINIZ, MARIANA O.; PORCHIA, BRUNA F. M. M.; SALES, NATIELY S.; MORENO, ANA CAROLINA R.; FERREIRA, LUIS C. S.. Bacillus subtilis spores as adjuvants for DNA vaccines. Vaccine, v. 33, n. 20, p. 2328-2334, . (11/13805-1)
APS, LUANA R. M. M.; TAVARES, MILENE B.; ROZENFELD, JULIO H. K.; LAMY, M. TERESA; FERREIRA, LUIS C. S.; DINIZ, MARIANA O.. Bacterial spores as particulate carriers for gene gun delivery of plasmid DNA. Journal of Biotechnology, v. 228, p. 58-66, . (11/13805-1)
MILAZZOTTO MALDONADO PORCHIA, BRUNA FELICIO; DE MELO MORAES APS, LUANA RAPOSO; RAMOS MORENO, ANA CAROLINA; DA SILVA, JAMILE RAMOS; SILVA, MARIANGELA DE OLIVEIRA; SALES, NATIELY SILVA; DOS SANTOS ALVES, RUBENS PRINCE; REILY ROCHA, CLARISSA RIBEIRO; SILVA, MATHEUS MOLINA; RODRIGUES, KARINE BITENCOURT; et al. Active immunization combined with cisplatin confers enhanced therapeutic protection and prevents relapses of HPV-induced tumors at different anatomical sites. International Journal of Biological Sciences, v. 18, n. 1, p. 15-29, . (11/13805-1, 20/13640-1, 11/20917-0, 17/21358-1, 13/15360-2, 15/16505-0, 18/08502-9)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
APS, Luana Raposo de Melo Moraes. Adjuvant effect of Bacillus subtilis spores co-administered with DNA vaccines.. 2013. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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Filed patent(s) as a result of this research project

ESPOROS DE BACILLUS SP., MÉTODO DE PREPARO DOS ESPOROS, MÉTODO DE PREPARO DE CARTUCHOS DE BIOBALiSTICA, E USOS DOS ESPOROS BR1020140096795 - Universidade de São Paulo (USP) . Julio Kravcuks Rozenfeld; Luana Raposo de Moraes Aps; Luis Calos de Souza Ferreira; Maria Teresa Moura Maly; Mariana de Oliveira Diniz; Milene Tavares Batista - April 2014, 23