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Effect of the Poly(Vinylidene-Trifluoroethylene)/Barium Titanate Membrane on the Bone Formation in Rat Cavariae Defects

Grant number: 11/13623-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): June 30, 2014
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Márcio Mateus Beloti
Grantee:Helena Bacha Lopes
Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):13/01622-5 - Large-scale analysis of the microRNAs expression in RNA samples obtained from newly formed bone tissue in contact with PTFE and PVDF membranes, BE.EP.MS


Tissue engineering principles are contributing to the development of membranes to be employed in a range of therapies. Specifically in dentistry, membranes are being used in different clinical situations like treatment with dental implants, augmentation of alveolar bone and repair of traumatic and/or pathologic bone defects. We carried out in vitro experiments to compare a novel poly(vinylidene fluoride-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT) membrane with polytetrafluorethylene (PTFE) one and the results indicate that the majority of parameters related to tissue regeneration was enhanced by P(VDF-TrFE)/BT membrane in three different cell lines (osteoblasts, fibroblasts and keratinocytes). In this context, this study aimed at comparing the in vivo bone tissue response to P(VDF-TrFE)/BT and PTFE (control) membranes. Bone defects (5-mm-diameter) will be prepared in rat cavariae distributed in three groups based on membrane use: (1) P(VDF-TrFE)/BT; (2) PTFE; (3) without membrane. At 4 and 8 weeks, the animals will be euthanized and the calvariae harvested to the following assays: (1) tomographic analysis (microCT); (2) histological and histomorphometric analyses; gene expression (real-time PCR) of the bone markers, runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OC), osteoprotegerin (OPG), receptor activator of nuclear factor-kappa B ligand (RANKL) and receptor activator of nuclear factor-kappa B (RANK). These bone markers will also be also detected in bone samples harvested from calvariae at the moment of the surgical procedure to implant the membranes aiming to compare the newly formed bone on membranes with previous calvarial bone. Five samples (n=5) for each membrane group and time and three (n=3) for the group without membrane will be evaluate. Statistical analysis will be selected according to the sampling distribution.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LOPES, HELENA B.; SANTOS, THIAGO DE S.; DE OLIVEIRA, FABIOLA S.; FREITAS, GILEADE P.; DE ALMEIDA, ADRIANA L. G.; GIMENES, ROSSANO; ROSA, ADALBERTO L.; BELOTI, MARCIO M.. Poly(vinylidene-trifluoroethylene)/barium titanate composite for in vivo support of bone formation. JOURNAL OF BIOMATERIALS APPLICATIONS, v. 29, n. 1, p. 104-112, . (11/13623-0, 11/10658-8)
LOPES, HELENA B.; FERRAZ, EMANUELA P.; ALMEIDA, ADRIANA L. G.; FLORIO, PEDRO; GIMENES, ROSSANO; ROSA, ADALBERTO L.; BELOTI, MARCIO M.. Participation of MicroRNA-34a and RANKL on bone repair induced by poly(vinylidene-trifluoroethylene)/barium titanate membrane. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, v. 27, n. 13, p. 1369-1379, . (13/01622-5, 11/13623-0, 11/10658-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LOPES, Helena Bacha. Effect of Poly(vinylidene-trifluoroethylene)/Barium Titanate Membrane on Bone Formation in Rat Calvaria Defects. 2014. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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