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Isolation and selection of thermophilic/thermotolerant filamentous fungi producer of cellulases and xylanases and application of the enzymatic extracts in sugarcane bagasse saccharification

Grant number: 11/12794-6
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): November 30, 2013
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal researcher:Daniela Alonso Bocchini
Grantee:Josiani de Cassia Pereira
Home Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated scholarship(s):12/20403-0 - Evaluation of ozone pretreatment influence in the saccharification of sugarcane bagasse by the enzymatic extract produced by a newly isolated thermophilic fungus, BE.EP.MS


The search for "clean" technologies to obtain products of industrial interest, involving the use of alternative raw materials, reduction on energy use and on effluents production, has been driven by environmental issues and are mostly economically advantageous . Within this context research on the use of lignocellulosic wastes in bioprocesses stand out, particularly in the cultivation of microorganisms producing cellulases, hemicellulases and ligninases. These enzymes have potential for various biotechnological applications, especially in recent years the disintegration and hydrolysis of lignocellulosic material to obtain fermentable sugars, which can be converted into second-generation ethanol. Thus, the prospect of microorganisms producing these enzymes is necessary not only for obtaining biocatalysts that have suitable characteristics for industrial processes to which they are intended, but also for knowledge and exploration of microbial biodiversity. Thus, this proposal involves the isolation of thermophilic/thermotolerant filamentous fungi producers of cellulases and xylanases, selection of the strain that stand out in the production of enzymes (taking into account not only the amount of enzyme produced, but also features like activity at high temperature, thermal stability, low inhibition by hydrolysis products and ethanol) and, finally, the evaluation of enzymatic extract effectiveness in the saccharification of in natura and pretreated with microwaves and glycerol sugarcane bagasse. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREIRA, JOSIANI DE CASSIA; TRAVAINI, RODOLFO; MARQUES, NATALIA PAGANINI; BOLADO-RODRIGUEZ, SILVIA; BOCCHINI MARTINS, DANIELA ALONSO. Saccharification of ozonated sugarcane bagasse using enzymes from Myceliophthora thermophila JCP 1-4 for sugars release and ethanol production. Bioresource Technology, v. 204, p. 122-129, . (11/19554-0, 11/12794-6)

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