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Gene expression profile in masticatory mucosa tissue of patients with history of chronic and aggressive periodontitis

Grant number: 11/03625-6
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): February 28, 2013
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Márcio Zaffalon Casati
Grantee:Tiago Taiete
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

This study will evaluate the gene expression profile of tissue from masticatory mucosa, without the influence of biofilm, in patients with a history of chronic and aggressive generalized periodontitis compared to individuals without a history of periodontitis, with the aim to identify possible constitutive alterations in gene expression and their mechanisms, in order to clarify the biological events responsible for the different susceptibilities of individuals to the periodontal disease development. It will be selected patients with history of chronic and aggressive generalized periodontitis, and individuals with no history of periodontitis, with indication for dental implants placement. Implant placement surgeries will be performed at Graduate Clinic of Piracicaba Dental School- UNICAMP. The removed tissue to adapt the healing abutment will be collected and stored in RNA stabilization reagent (Invitrogen RNAlater ®). Tissue samples will be processing for RNA extraction, cDNA synthesis, for the subsequent preparation of hybridization reactions on microarrays, which will be assessed the expression of 44049 genes, covering the whole genome. Results will be analyzed to identify gene expression profiles of each group of patient, allowing the description of genes that had differential expression in each group as well as classes of genes that had differential modulation, and of possible pathways that these genes are related. The top 20 genes that had different expression between patient groups will be analyzed by real-time PCR, to confirm the results. Statistical analysis will be performed by ANOVA / Tukey to determine whether there are differences gene expression between groups.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TAIETE, TIAGO; VIANA CASARIN, RENATO CORREA; SILVERIO RUIZ, KARINA GONZALES; NOCITI, JR., FRANCISCO HUMBERTO; SALLUM, ENILSON ANTONIO; CASATI, MARCIO ZAFFALON. Transcriptome of healthy gingival tissue from edentulous sites in patients with a history of generalized aggressive periodontitis. Journal of Periodontology, v. 89, n. 1, p. 93-104, . (11/03625-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
TAIETE, Tiago. Tecnologias moleculares no estudo das doenças periodontais. 2013. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.

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