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Evaluation of T lymphocyte subsets in blood and cerebrospinal fluid in dogs with visceral leishmaniasis: correlation with brain lesions

Grant number: 11/03504-4
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): August 31, 2013
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal Investigator:Gisele Fabrino Machado
Grantee:Fernanda Grecco Grano
Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Associated scholarship(s):12/21129-9 - Real-Time PCR analysis in the brain of naturally infected dogs by Leishmania chagasi, BE.EP.MS


The infection caused by Leishmania spp. results in a disease that presents a variety of clinical manifestations both in humans and dogs. These manifestations can range from observation of asymptomatic infected dogs to disorders that can result in the death of the host. Chronically infected dogs may show neurological symptoms, however there are few reports that characterize the lesions and elucidate the pathogenesis of visceral leishmaniasis within the central nervous system. In previous projects, it was possible to verify the presence of inflammatory infiltrate in the brain of dogs with leishmaniasis, mainly composed of CD3+ T lymphocytes and an increase in the intensity of labeling of GFAP+ astrocytes and RCA-1+ microglia. In order to evaluate the mechanisms involved in the pathogenesis of the brain lesions in these dogs, we propose to characterize the T lymphocytes subsets in the blood and cerebrospinal fluid. T cells will be evaluated by flow cytometry and the phenotypes evaluated will be CD3+, CD4+, CD8+, TCR ±² and TCR ³´. Histological sections of the brain of the same dogs also will be obtained for evaluation and characterization of brain lesions.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GRANO, FERNANDA G.; MELO, GUILHERME D.; BELINCHON-LORENZO, SILVIA; GOMEZ-NIETO, LUIS C.; MACHADO, GISELE F.. First detection of Leishmania infantum DNA within the brain of naturally infected dogs. Veterinary Parasitology, v. 204, n. 3-4, p. 376-380, . (11/03504-4, 12/10415-0, 12/21129-9)
GRANO, FERNANDA G.; SILVAA, JOSE EDUARDO DOS S.; MELO, GUILHERME D.; PEROSSO, JULIANA; LIMA, VALERIA M. F.; MACHADO, GISELE F.. T lymphocyte immunophenotypes in the cerebrospinal fluid of dogs with visceral leishmaniasis. Veterinary Parasitology, v. 232, p. 12-20, . (11/03504-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GRANO, Fernanda Grecco. Imunofenótipos de linfócitos T no sangue e no liquor de cães com leishmaniose visceral: correlação com as lesões encefálicas. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária. Araçatuba Araçatuba.

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