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Phylogeography, population genetics and species delimitation on the Cattleya coccinea complex (Orchidaceae)

Grant number: 11/18751-7
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): February 01, 2012
Effective date (End): February 28, 2015
Field of knowledge:Biological Sciences - Genetics - Plant Genetics
Principal Investigator:Elizabeth Ann Veasey
Grantee:Jucelene Fernandes Rodrigues
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil


The reconstruction of the complex evolutionary history of species is not a simple task as taxonomically problematic groups are often a result of recent speciation events or rapid speciation. Although orchids belonging to the 'Cattleya coccinea' complex have clear diagnostic morphological characters that allow their identification, the delimitation of its seven currently recognized species is problematic. Results of a preliminary study based on ISSR markers and plastid DNA sequences corroborate previous observations on unclear patterns of morphological variation among taxa currently recognized. Within this view, the objective of this study is to understand patterns of diversity between and within populations of the 'Cattleya coccinea' complex from a wider geographic and genetic sampling than the previous study, in order to identify distinct evolutionary lineages and review current delimitation of species for the entire 'Cattleya coccinea' complex. Specifically, this study aims to: (1) measure levels of genetic differentiation within and among populations of species currently belonging to the 'Cattleya coccinea' complex considering the full range of morphological and geographical variation, microsatellite markers and plastid sequences, (2) infer important historical demographic processes, and ecological and evolutionary mechanisms generating the structure patterns observed, and (3) discuss potential evolutionary and conservationist implications of the results for the orchids from the Brazilian Atlantic Forest. With this purpose on mind we intend to develop phylogeographic and population genetic studies based on plastidial DNA sequences and microsatellite markers. Collections will be held in 11 locations in the states of Rio Grande do Sul, Santa Catarina, São Paulo, Rio de Janeiro, Minas Gerais and Espirito Santo, covering all the geographical and morphological distribution observed for the 'Cattleya coccinea' complex. For the plastid sequences analysis, two non-coding sequences already identified as polymorphic will be used, while other primers will be tested to check for the occurrence of additional polymorphic regions. For the microsatellite analysis eight primers already identified as polymorphic will be used and ten extra primers will be tested. With this data, phylogenetic analysis, diversity and population genetic structure analysis will be made, and finally phylogeographic analysis will be held in order to meet the objectives proposed in the study. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RODRIGUES, JUCELENE F.; VAN DEN BERG, CASSIO; ABREU, ALUANA G.; NOVELLO, MARIANA; VEASEY, ELIZABETH A.; OLIVEIRA, GIANCARLO C. X.; KOEHLER, SAMANTHA. Species delimitation of Cattleya coccinea and C-mantiqueirae (Orchidaceae): insights from phylogenetic and population genetics analyses. PLANT SYSTEMATICS AND EVOLUTION, v. 301, n. 5, p. 1345-1359, . (11/18532-3, 11/18751-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
RODRIGUES, Jucelene Fernandes. Phylogenetic and phylogeographic relationships of the species of the complex \'Cattleya coccinea\' (Orchidaceae). 2015. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.

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