Salmonella enteritidis (SE) has public health importance, since it is responsible for cases of foodborne disease associated with consumption of food from poultry origin. In this project, the study of mutant strains with deletions in genes involved in vitamin B12 biosynthesis (cob operon will be complemented with the study of strains containing deletions in genes responsible for producing the enzyme-propanediol dehydratase (pdu operon) that converts 1,2-propanediol to propionaldehyde and uses vitamin B12 as its cofactor. In order to do this, two mutant strains of SE will be constructed: one strain with pduC, pduD and pduE genes defectives (SE pduCpduDpduE) and another one with deletions in genes of the operons cob and pdu (SE cobScbiApduCpduDpduE). It will be analyzed the pathology of these mutants, comparing them with a wild type strain (SE), assessing the systemic infection, mortality and faecal shedding. Moreover, the immune response will be characterized by analyzing populations of lymphocytes (CD4/CD8) in liver and caecal tonsils and by measurement of expression of genes responsible for the of IL-1², IL-6, MIP, CXCLi1, CXCLi2 and IL-22 in spleen ,the wall of the caeca and caecal tonsils in order to assess the pathogenicity and elucidate aspects of bacterial survival mechanisms in the host.
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