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Infection of birds (Gallus Gallus domesticus) with Salmonella enterica subsp. enterica serovar Enteritidis containing deletions in cobs, cbiA, pduC, pduD and pduE genes: evaluation of intestinal colonization, systemic invasion and immune responses

Grant number: 11/20355-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2012
Effective date (End): April 30, 2015
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal researcher:Angelo Berchieri Junior
Grantee:Janine Denadai
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Salmonella enteritidis (SE) has public health importance, since it is responsible for cases of foodborne disease associated with consumption of food from poultry origin. In this project, the study of mutant strains with deletions in genes involved in vitamin B12 biosynthesis (cob operon will be complemented with the study of strains containing deletions in genes responsible for producing the enzyme-propanediol dehydratase (pdu operon) that converts 1,2-propanediol to propionaldehyde and uses vitamin B12 as its cofactor. In order to do this, two mutant strains of SE will be constructed: one strain with pduC, pduD and pduE genes defectives (SE ”pduC”pduD”pduE) and another one with deletions in genes of the operons cob and pdu (SE ”cobS”cbiA”pduC”pduD”pduE). It will be analyzed the pathology of these mutants, comparing them with a wild type strain (SE), assessing the systemic infection, mortality and faecal shedding. Moreover, the immune response will be characterized by analyzing populations of lymphocytes (CD4/CD8) in liver and caecal tonsils and by measurement of expression of genes responsible for the of IL-1², IL-6, MIP, CXCLi1, CXCLi2 and IL-22 in spleen ,the wall of the caeca and caecal tonsils in order to assess the pathogenicity and elucidate aspects of bacterial survival mechanisms in the host.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PAIVA, J. B.; DENADAI, J.; ALMEIDA, A. M.; BARROW, P. A.; BARBOSA, F. O.; ALVES, L. B. R.; SARAIVA, M. M. S.; OLIVEIRA, C. J. B.; JUNIOR, A. BERCHIERI; NETO, O. C. FREITAS. Evaluation of propanediol and cobalamin metabolism in the intestinal colonization and systemic invasion of Salmonella Enteritidis in laying hens. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 72, n. 6, p. 2391-2396, NOV-DEC 2020. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.