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Determination of inorganic composition and metallic species bioavailable fraction in cocoa-based food

Grant number: 11/21023-3
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): March 01, 2012
Effective date (End): August 31, 2015
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Solange Cadore
Grantee:Rafaella Regina Alves Peixoto
Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated scholarship(s):13/21962-5 - Study of the bioavailability of metallic elements from cocoa products, BE.EP.DD


The objective of this project is the determination of the inorganic composition of cocoa-based food and the establishment of sample preparation methods for in vitro determination of metallic species bioavailability. For the bioavailability study it will be use a method of gastrointestinal digestion in vitro, where digestion will simulate the steps that occur in the mouth, stomach and intestine and, still, a step of dialysis. The quantification of the analytes, total concentration in the food and the bioavailable fraction, will be held using spectrometric methods of analysis, such as Optical Emission with Inductively Coupled Plasma Spectrometry (ICPOES) and Graphite Furnace Atomic Absorption Spectrometry (GFAAS), depending on the content of the analyte to be determined. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEIXOTO, RAFAELLA R. A.; DEVESA, VICENTA; VELEZ, DINORAZ; LUISA CERVERA, M.; CADORE, SOLANGE. Study of the factors influencing the bioaccessibility of 10 elements from chocolate drink powder. Journal of Food Composition and Analysis, v. 48, p. 41-47, MAY 2016. Web of Science Citations: 15.
VILLA, JAVIER E. L.; PEIXOTO, RAFAELLA R. A.; CADORE, SOLANGE. Cadmium and Lead in Chocolates Commercialized in Brazil. Journal of Agricultural and Food Chemistry, v. 62, n. 34, p. 8759-8763, AUG 27 2014. Web of Science Citations: 24.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
. Metallic elements in chocolate drink powder : total contents and bioaccessible and bioavailable fractions. 2015. 131 f. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Química.

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