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Relationship between 2 acyl coa:diacylglycerol acyltransferase enzyme and NAFLD prognostic on chronic overload nutritional condition

Grant number: 11/18644-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2012
Effective date (End): December 31, 2012
Field of knowledge:Health Sciences - Nutrition - Nutrition Biochemistry
Principal researcher:André Ferreira Do Nascimento
Grantee:Aline de Oliveira Martins
Home Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


Recent evidences suggest NAFLD is an important risk factor for cardiovascular disease. However, it is unknown if the cardiovascular risk is present in NAFLD with pure steatosis or necro-inflammation characteristics. Results demonstrated that a steatosis block might be a risk factor for NAFLD progression, which derivate from lesser activity of 2 acyl CoA:diacylglycerol acyltransferase enzyme (DGAT2). The time-dependent relationship of this event has been demonstrated. Thus, the objective of this study will be evaluating the relationship between 2 acyl CoA:diacylglycerol acyltransferase enzyme and NAFLD prognostic on chronic overload nutritional condition. Male Wistar rats from São Paulo State University Animal Center - UNESP - Botucatu/SP will be fed with normal chow or hipercaloric diet for 6, 12 and 24 weeks (C6, C12, C24, H6, H12, H24; n=15 animals/group). NAFLD indicators will be evaluated, such as, steatosis and inflammatory process. Hepatic free fatty acid pool will also be determined. Hepatic DGAT2 levels will be evaluated for protein and gene expression by Western Blot and Real Time PCR, respectively. Serum biochemistry profile will also be analyzed. Data will be reported as descriptive measures of position and variability. Comparisons between groups will be performed using two-way ANOVA for independent groups and complemented by post hoc Tukey test. A 5% significance level will be adopted. (AU)

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