Evaluation of the effects of AsGaAl laser on the proliferation of muscle cells during the differentiation process

Abstract

There is considerable interest in establishing therapies that provide a better, faster muscle repair process. A growing body of evidence suggests that low-level laser therapy (LLLT) promotes skeletal muscle regeneration, but its effectiveness in the treatment of injury remains questionable and its therapeutic potential is not fully established. The aim of the present study is to evaluate the effects of low-level AsGaAl 780nm laser (Twin-Laser ®, MMOptics) on the proliferation of C2C12 skeletal muscle precursor cells during the differentiation process. C2C12 myoblasts will be cultured in Dulbecco's modified Eagle medium containing 10% fetal bovine serum and will be induced to differentiate by the addition of 2% horse fetal serum before initiating LLLT. The laser parameters will be the power of 10 mW, and energy density of 3 and 5 J/cm2 for 12 and 20 seconds. Myoblast proliferation will be evaluated after 24, 48, and 96 hours after laser irradiation through a MTT assay. A non-irradiated group will serve as the control. The results will be statistically analyzed using ANOVA/Tukey (p < 0.05) to determine differences between groups.(AU)