The role of galectin-3 in the migration and recruitment of mast cells to the peritoneal cavity

Abstract

Mast cells are immunoregulatory cells that participate in allergy and inflammation. In order to participate in these processes, immature mast cells leave the bone marrow and migrate to peripheral sites. Accordingly, cell migration is an essential element in the recruitment of mast cells. Galectins are proteins that recognize ²-galactosides and participate in cell migration, adhesion, and proliferation, among other processes. The aim of this project is to investigate if galectin-3 is involved in mast cell recruitment to the peritoneal cavity, in response to allergic stimuli. The mast cell population recruited to the peritoneal cavity will be investigated, after an allergic stimulus. The expression of molecules related to cell migration, such as cortactin, will be analyzed in mast cells isolated from peritoneal lavage and also in the mesentery. Other molecules such as RhoA, CDC 42, and Rac1, all involved in the formation of lamellipodia and filopodia, will also be studied. The results from this study may contribute to a better understanding of the role of galectin-3 in the mast cell migration. (AU)