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Model animal of augmentation related to movement disorders of sleep

Grant number: 11/12540-4
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2011
Effective date (End): September 30, 2012
Field of knowledge:Biological Sciences - Pharmacology - General Pharmacology
Principal Investigator:Marco Túlio de Mello
Grantee:Welinton Alessandro Oliveira de Almeida
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Restless Legs Syndrome (RLS) is a neurological disorder that is characterized by the need to move the legs, associated with paresthesia, uncomfortable sensations, and motor restlessness. During the long-term treatment with dopamine was observed an unusual phenomenon called Augmentation, was considered a consequence of treatment. Objective: Identifying an animal model of augmentation undergoing pharmacological treatment with a dopamine agonist. Methods: Will be used 20 male Wistar rats (3 months). The animals will be distributed in the treatment group (TTO) - of 10 rats, and the control group (CTRL) - of 10 rats. Initially, the animals will perform an adaptation to the open field (10 minutes). After the adjustment period will be started the treatment, where animals receive the drug or saline daily, always at the same time, for 28 days. Will be held tests in an open field on days 1, 7, 14, 21, and 28 of treatment. Pramipexole (PPX - 0.1 mg/kg) and saline (0.1 mg/kg) will be injected into the peritoneal cavity 15 minutes before the start of the period in which animals will be evaluated in the open field. The treatment will take place around the same time. Will be determined the protein expression of Tyrosine Hydroxylase (TH) and D1 and D2 receptors by western blot.(AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
OLIVEIRA DE ALMEIDA, WELINTON ALESSANDRO; ESTEVES, ANDREA MACULANO; DE ALMEIDA-JUNIOR, CANUTO LEITE; LEE, KIL SUN; FRANK, MIRIAM KANNEBLEY; MARIANO, MELISE OLIVEIRA; FRUSSA-FILHO, ROBERTO; TUFIK, SERGIO; DE MELLO, MARCO TULIO. The effects of long-term dopaminergic treatment on locomotor behavior in rats. SLEEP SCIENCE, v. 7, n. 4, p. 6-pg., . (98/14303-3, 11/12540-4)

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