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Analysis and Gene Expression of PKS II and carbohydrases from metagenomic libraries

Grant number: 11/10981-3
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2011
Effective date (End): January 31, 2015
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Eliana Gertrudes de Macedo Lemos
Grantee:Elisângela Soares Gomes Pepe
Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil


Polyketide are varied secondary metabolites present in some species of micro-organisms and plants. They are part of the composition of microbial spore pigments and antibiotics, and actuan in physiological mechanisms of differentiation in the face of environmental adverse factors. They are synthesized by the enzyme complexes named Polyketide Synthases (PKSs I, II and III). Previously had carried out a prospection of new clusters for gene PKSs II in a metagenomic library of 9320 clones (FAPESP, CI process: 07/53708-0, CNPq, Master process: 130454/2010-9). An environmental insert DNA with 22,988 base pairs was found, sequenced and subjected to prediction functional of ORFs by homology and three-dimensional structure modeling "in silico". The results of these tests allowed verify the presence of homology to ABC transporter genes; Minimal PKS, cyclases; aromatases; hydroxylases, mono-oxygenases; O- methyl-transferases; carbohydrases; Peptidases; acyl transferase; LacI and LuxR genic transcription regulators, among others. There arestrong indications that it is a new pathway of polyketides pigments. The insert also contain some genes related to complex carbohydrates degradation (cellulose / chitin / lignin). It is proposed in this research project the development of experiments in genic expression (homologous and heterologous) of the biosynthetic's PKS core present in this metagenomic insert, and heterologous expression of carbohydrases of industrial interest.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MONTEIRO LIMA, NATALIA SARMANHO; GOMES-PEPE, ELISANGELA SOARES; CAMPANHARO, JOAO CARLOS; DE MACEDO LEMOS, ELIANA GERTRUDES. Broad thermal spectrum metagenomic laccase with action for dye decolorization and fentin hydroxide treatment. AMB EXPRESS, v. 12, n. 1, p. 14-pg., . (11/10981-3)
LIMA, NATALIA SARMANHO MONTEIRO; GOMES-PEPE, ELISANGELA SOARES; KOCK, FLAVIO VINICIUS CRIZOSTOMO; COLNAGO, LUIZ ALBERTO; LEMOS, ELIANA GERTRUDES DE MACEDO. Dynamics of the role of LacMeta laccase in the complete degradation and detoxification of malachite green. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 39, n. 5, p. 13-pg., . (19/23014-3, 19/20825-0, 11/10981-3)
GOMES-PEPE, ELISANGELA SOARES; MACHADO SIERRA, ELWI GUILLERMO; PEREIRA, MARIANA RANGEL; LUQUE CASTELLANE, TEREZA CRISTINA; DE MACEDO LEMOS, ELIANA GERTRUDES. Bg10: A Novel Metagenomics Alcohol-Tolerant and Glucose-Stimulated GH1 SS-Glucosidase Suitable for Lactose-Free Milk Preparation. PLoS One, v. 11, n. 12, . (11/10981-3)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PEPE, Elisângela Soares Gomes. Análise e expressão de genes de PKS II e de carboidrases provenientes de bibliotecas metagenômicas. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.

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