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Analysis of subgingival microbiota diversity and serum cytokine and gingival fluid profile related to glycemic control in subjects with diabetes mellitus type II

Grant number: 11/10057-4
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): December 01, 2011
Effective date (End): March 09, 2015
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Marcia Pinto Alves Mayer
Grantee:Priscila Larcher Longo
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):13/20537-9 - Analysis of data from 16SrRNA sequencing generated by high throughput of subgingival biofilm samples from diabetic patients, BE.EP.PD


There is a bidirectional relationship between periodontitis and diabetes mellitus type II, where the treatment of periodontal infection can be considered part of diabetes treatment, whereas glycemic control is a prerequisite for successful periodontal therapy. It is unknown inflammatory markers and subgingival microbiota of periodontitis cases associated with diabetes with glycemic imbalance of chronic periodontitis cases from other diabetics and nondiabetics, although these have different severity and prognosis. This study aims to test the hypothesis that the subgingival microbiota of diabetic patients without glycemic balance differs from the microflora of diabetic patients with glicemic control and non-diabetic controls, reflecting and / or being a reflection of the exacerbated inflammation in cases of failure of glycemic balance. It will be selected 30 patients with periodontitis, being 10 without diabetes, 10 diabetics with glycemic control and 10 diabetic patients with poor glycemic control. Patients will undergo medical examinations, blood collection, clinical examination and sampling of subgingival plaque and gingival fluid. The diversity of subgingival microbiota will be analyzed by 16S rRNA genomic libraries and biofilm samples from shallow and deep sites will be analyzed for levels of known pathogens and organisms selected from the diversity data by quantification of RNA with primers (ROQT). Profile of cytokines and mediators involved in bone resorption will be analysed in samples of gingival fluid and serum. These data will justify longitudinal studies in which specific markers for periodontitis in diabetic patients are determined and their relationship to balance glycemic and effect of periodontal treatment will be evaluated.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LONGO, PRISCILA L.; DABDOUB, SHAREEF; KUMAR, PURNIMA; ARTESE, HILANA P. C.; DIB, SERGIO A.; ROMITO, GIUSEPPE A.; ALVES MAYER, MARCIA PINTO. Glycaemic status affects the subgingival microbiome of diabetic patients. JOURNAL OF CLINICAL PERIODONTOLOGY, v. 45, n. 8, p. 932-940, . (13/20537-9, 11/10057-4, 11/18618-5)
HUI ZI, MARCELAYANG; LONGO, PRISCILA LARCHER; BUENO-SILVA, BRUNO; ALVES MAYER, MARCIA PINTO. Mechanisms involved in the association between periodontitis and complications in pregnancy. FRONTIERS IN PUBLIC HEALTH, v. 2, p. 13-pg., . (14/10549-2, 11/10057-4, 12/01500-4)

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