There is a bidirectional relationship between periodontitis and diabetes mellitus type II, where the treatment of periodontal infection can be considered part of diabetes treatment, whereas glycemic control is a prerequisite for successful periodontal therapy. It is unknown inflammatory markers and subgingival microbiota of periodontitis cases associated with diabetes with glycemic imbalance of chronic periodontitis cases from other diabetics and nondiabetics, although these have different severity and prognosis. This study aims to test the hypothesis that the subgingival microbiota of diabetic patients without glycemic balance differs from the microflora of diabetic patients with glicemic control and non-diabetic controls, reflecting and / or being a reflection of the exacerbated inflammation in cases of failure of glycemic balance. It will be selected 30 patients with periodontitis, being 10 without diabetes, 10 diabetics with glycemic control and 10 diabetic patients with poor glycemic control. Patients will undergo medical examinations, blood collection, clinical examination and sampling of subgingival plaque and gingival fluid. The diversity of subgingival microbiota will be analyzed by 16S rRNA genomic libraries and biofilm samples from shallow and deep sites will be analyzed for levels of known pathogens and organisms selected from the diversity data by quantification of RNA with primers (ROQT). Profile of cytokines and mediators involved in bone resorption will be analysed in samples of gingival fluid and serum. These data will justify longitudinal studies in which specific markers for periodontitis in diabetic patients are determined and their relationship to balance glycemic and effect of periodontal treatment will be evaluated.
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