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AHNAK regulates formation and exchange of extracellular vesicles between breast tumor cells and fibroblasts

Grant number: 11/09472-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2011
Effective date (End): July 31, 2015
Field of knowledge:Biological Sciences - Morphology - Cytology and Cell Biology
Principal researcher:Vanessa Morais Freitas
Grantee:Thaiomara Alves Silva
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:10/07699-1 - Protease ADAMTS1 influencing breast cancer behavior and microenvironment , AP.JP


The successful development of tumors is not only dependent on cell mutations, but also driven by the tissue microenvironment; relies on interaction of cells and their surrounding stroma. Some cell types release vesicular structures into the extracellular space that would be involved in cellular communication and tumor progression. The aim of this study was to analyze vesicle-mediated interactions between tumor cells and normal fibroblasts. Tumor cells were plated above fibroblasts monolayer and both loaded with different vital dyes. Our results evidenciated presence and exchange of vesicles between breast tumor cells and fibroblasts in co-culture. Vesicles isolated showed heterogeneous sizes. Tumor cell showed more vesicles than normal cells. These vesicles were composed of AHNAK and Annexins proteins. The protein AHNAK was detected in exchanged vesicles and was increased in tumors when compared to normal breast tissues. AHNAK could represent a vesicle structural molecule that would influence breast tumor biology. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA, THAIOMARA A.; SMUCZEK, BASILIO; VALADAO, IURI C.; DZIK, LUCIANA M.; IGLESIA, REBECA P.; CRUZ, MARIO C.; ZELANIS, ANDRE; DE SIQUEIRA, ADRIANE S.; SERRANO, SOLANGE M. T.; GOLDBERG, GARY S.; et al. AHNAK enables mammary carcinoma cells to produce extracellular vesicles that increase neighboring fibroblast cell motility. ONCOTARGET, v. 7, n. 31, p. 49998-50016, . (13/07467-1, 10/07699-1, 15/00544-6, 11/09472-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Thaiomara Alves. AHNAK regulates the formation and exchange of extracellular vesicles from breast tumor cells and fibroblasts.. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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