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Vat (vacuolating autotransporter toxin) produced by APEC: internalization, intracellular translocation and cytotoxic activity in avian cells

Grant number: 11/03586-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2011
Effective date (End): July 31, 2013
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Tomomasa Yano
Grantee:Natália Galdi Quel
Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


Escherichia coli isolated from avian cellulits lesions in broiler chickens produce a cytotoxin called Vat (vacuolating autotransporter toxin)that causes intense vacuolization in avian cells, but not in mammalian cells. Previous studies have shown that Vat induced, in broilers, releasing inflammatory cytokines TNF-± and IL-10. Moreover, histological analysis of lesions induced experimentally by Vat, in broilers, showed microscopic changes in subcutaneous, adipose and connective tissues, including inflammation signs. These data indicate the role of Vat in the development of avian cellulitis. In addition, the study of the intracellular activity of Vat: internalization, intracellular translocation and structural changes in chicken embrio fibroblasts (CEF) may help to elucidate the specificity of Vat in CEF and not to mammalian cells. It also aims to relate the specificity of anti-Vat antibody against biological activities of other vacuolating cytotoxins. The results may contribute to understanding the mechanisms of development of avian cellulitis in broiler chickens. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ANNELIZE ZAMBON BARBOSA ARAGÃO; NATÁLIA GALDI QUEL; PAULO PINTO JOAZEIRO; TOMOMASA YANO. Escherichia coli vacuolating factor, involved in avian cellulitis, induces actin contraction and binds to cytoskeleton proteins in fibroblasts. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 27, . (11/03586-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
QUEL, Natália Galdi. Escherichia coli Vacoulating Factor (ECVF) as a factor associated to avian cellulitis.. 2014. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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