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The role of ER stress in cell death of neutrophils from diabetic rats

Grant number: 10/12818-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2011
Effective date (End): September 30, 2013
Field of knowledge:Biological Sciences - Physiology - General Physiology
Principal Investigator:Tatiana Carolina Alba Loureiro
Grantee:Wilson Mitsuo Tatagiba Kuwabara
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):12/05349-9 - Possible evidences of ER and mitochondria participation in neutrophil HL-60 differentiated death under hyperglycemia condition, BE.EP.MS

Abstract

Nowadays, the ER stress has been investigated in cell death process. This cellular compartment can be under stress condition because of protein formation alterations,can participate in ROS production, as well as NADPH oxidase and mitochondria, and promote a reaction cascate from reticulum stress. The aim of this project is to investigate the participation of ER stress in cell death process in neutrophils from diabetic rats. Some parameters will be analysed: the ER in neutrophils by fluorescence microscopy; the ROS production by NADPH oxidade, mitochondria and ER using the amplex red techinique; the caspases 3,4,7 and 12 activities; the content and possible activation or clivages of Bip, IRE1, PERK, ATF-6, ATF-4, eIF2 and ERO1 by western blotting and the expression of the genes Bip, IRE-1, PERK, ATF-6, XBP-1 and CHOP by real time. This study focuses to understand the participation of ER in cell death process of neutrophils from diabetic rats and the possible interation of ER with mitochondria and NADPH oxidase. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TATAGIBA KUWABARA, WILSON MITSUO; CURI, RUI; ALBA-LOUREIRO, TATIANA CAROLINA. Autophagy Is Impaired in Neutrophils from Streptozotocin-Induced Diabetic Rats. FRONTIERS IN IMMUNOLOGY, v. 8, . (15/03175-1, 10/12818-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
KUWABARA, Wilson Mitsuo Tatagiba. The role of the endoplasmatic reticulum in the process of death of neutrophils from diabetic rats.. 2013. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

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