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Use of c-DNA microarrays to detect alterations in gene transcription in mast cell tumors in dogs

Grant number: 11/04038-7
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2011
Effective date (End): February 28, 2013
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal Investigator:Mirela Tinucci Costa
Grantee:Paulo César Jark
Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Mast cell tumours are the most common cutaneous malignant tumours in the dogs. Although there are several prognostic factors, the clinical and biological behavior of this tumor is highly variable, making the choice of the best treatment to be used, a challenge. Thus, the use of molecular techniques to evaluate a large number of genes involved in the neoplastic process, as it can be a valuable tool in determining prognosis and the election of candidate genes to molecular targeted therapy in the future. One way to identify the genes associated with the development or tumor progression is the investigation of numerical and structural changes in DNA isolated from tumor cells using the technique of CGHarray (comparative genomic hybridization).This study will be designed with the objective of comparing changes in genomic copy number (gains / losses) in canine cutaneous mast cell tumors of animals that survive a period less than six months (SV <6), those with survival greater than one year (SV> 12). For it will be selected 10 dogs with cutaneous mast cell tumor. The technique CGHarray will be held after the extraction of DNA and is used to Canine Genome CGH Microarray platform 4x180 (ID-252552-Agilent). Data analysis is performed using the program Nexusversion 5.0 (Biodiscovery). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
JARK, PAULO C.; MUNDIN, DEBORAH B. P.; DE CARVALHO, MARCIO; FERIOLI, RAQUEL B.; ANAI, LETICIA A.; MARCHI, FABIO A.; ROGATTO, SILVIA R.; LAUFER-AMORIM, RENEE; TINUCCI-COSTA, MIRELA. Genomic copy number variation associated with clinical outcome in canine cutaneous mast cell tumors. Research in Veterinary Science, v. 111, p. 26-30, . (11/09224-3, 11/04038-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
JARK, Paulo César. Alterações no número de cópias genômicas em mastocitomas cutâneos caninos. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.

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