Serum amyloid A (SAA) is an acute-phase protein and important inflammatory response mediator. In the acute-phase responses it is produced in high amounts and in chronic inflammatory disease there is a constant and tenuous production. Moreover, it is known that tumor cells produce SAA and its production is correlate positively with tumor malignancy. Recently, we demonstrated that SAA stimulated the proliferation of two glioma cell lines. With respect to migration and invasion, we observed that for one line there is an increase in migration and chemotaxis while there is an inhibition for the other one. Since the importance of proliferation, chemotaxis and migration phenomena for metastasis development, it is important to recognize patterns of SAA action in tumor cells. For this, we are going to evaluate the SAA effects on proliferation, migration and invasion in melanoma cell lines. Additionally, we have established a collaboration with Prof. Gilles Landman (UNIFESP), in which we are going to analyze the SAA gene and imunohistochemistry expression in primary cultures of melanoma cells from biopsies clinical samples and will made a correlation with tumor malignancy. Whereas the tridimensional (3D) organization is essential for tumor development studies, we are going to use a model of tridimensional skin in which we will observe the melanoma growth and the extracellular matrix proteins behavior, with and without SAA, in collaboration with Prof. Silvya Engler (FCF-USP). In this study, we will demonstrate the direct action of SAA in melanoma cells and its effects in the development local of the tumor. Together our other results, we want to recognize a pattern, or not, for SAA action in tumors.
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