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Caveolin-1 gene overexpression and interference: consequences for cell differentiation from human dental stem cells

Grant number: 11/04342-8
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2011
Effective date (End): April 30, 2013
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Katiúcia Batista da Silva Paiva
Grantee:Luiz Henrique Santos Silva
Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:10/08918-9 - Gene-modified-tissue engineering of bone: overexpression and inhibition of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs and RECK) by osteoblasts from human mesenchymal stem cells seeded on collagen-chitosan, AP.JP


Caveolina-1 (Cav-1) is an essential protein of caveolae formation and acts as scaffolding protein, organizing macromolecular complexes to enable highly localized and efficient intracellular signaling. Cav-1 interacts with various signaling proteins and growth factors, and also important role in regulating enzymes matrix metalloproteinases (MMPs) and their inhibitors (TIMPs and RECK), which regulate the rate of extracellular matrix (ECM) components degradation, essential for embryonic development and maintenance in adult tissue. Our group has been studying the participation of these proteins in the normal development of mineralized tissues (bone and dental) as well as associated to craniofacial cavity pathologies (periodontal disease, lip/cleft palate-associated polymorphism and tooth agenesis). Furthermore, we evaluated their role in ECM remodeling in potential biomaterials for dentistry and orthopedic application. Recently, for the first time, we demonstrated Cav-1 distribution during odontogenesis, palatogenesis, endochondral and intramembranous ossification in mice embryo. The quest for knowledge and understanding the regulatory mechanism to control self-renewal and differentiation of stem cells is one of the main aspects for the use of these in the context of Cell Therapy and Tissue Bioengineering. Specifically in the search for bone and tooth regeneration, dental stem cell (DSC) is becoming increasingly the focus of many researches in this area and is part of stem cells studied at NUCEL. Thus, this project intends to verify if: I) changing gene expression, either by overexpression or inhibition (interference), of Cav-1 (Cav-1± e Cav-1²), can modulate DSCs differentiation induction in vitro and II) if MMPs and their inhibitors (TIMPs and RECK) are regulated in these processes. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Luiz Henrique Santos. Gene expression of extracellular matrix and cell membrane molecules during cellular differentiation from human dental pulp stem cells. 2014. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.

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