Microbiological and molecular characterization, and somatic cell count by flow cytometry (Somacount 300) and microscopic count (PRESCOTT and breed, 1910) from mastitic of caprine milk

Abstract

The goat mastitis is defined as inflammation of the mammary gland and causes physical changes, chemical, microbiological and cellularity of milk. It is one of the main bottlenecks in the dairy industry, causing a reduction in production, depreciation of the product and its derivatives, discarded early female deaths of newborns and risk to public health. In the initial project would be evaluated in 1200 samples of milk from 20 goat farms located in the states of Sao Paulo and Minas Gerais, considering about 30 goats per Capril. However, due to economic problems, many farmers left the activity or livestock decreased dramatically, which is hampering the harvest. The criterion for inclusion of animals per property rose to at least 20 goats per farm, considering also the minimum number of 10 properties. Our objectives are: microbiologic and biochemical characterization of coagulase positive staphylococci (S. aureus, S. intermedius and S. hyicus) in the milk of goats with mastitis, to compare the method of flow cytometry (Somacount 300 ®) used for counting somatic cell count (SCC) in bovine milk with the direct microscopic count (Prescott and Breed, 1910) and establish a correlation between the methods with the results obtained from the California Mastitis Test (CMT) which will be a screening test for subclinical mastitis; determine the presence of Staphylococcus aureus (S. aureus), Streptococcus agalactiae (S. agalactiae), and Escherichia coli (E. coli) in milk samples from lactating goats compound independent of the result of CMT and milk samples from all animals (tank), following the diagnosis by multiplex PCR (mPCR), beyond the research of other pathogens in microbiological examination and analysis of risk factors for the occurrence of mastitis, involving the laboratory test results with the variables adopted in the health management properties, obtained by applying epidemiological questionnaire. (AU)