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Microbiological and molecular characterization, and somatic cell count by flow cytometry (Somacount 300) and microscopic count (PRESCOTT and breed, 1910) from mastitic of caprine milk

Grant number: 10/17254-7
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2011
Effective date (End): June 30, 2013
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:Helio Langoni
Grantee:Gustavo Puglia Machado
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


The goat mastitis is defined as inflammation of the mammary gland and causes physical changes, chemical, microbiological and cellularity of milk. It is one of the main bottlenecks in the dairy industry, causing a reduction in production, depreciation of the product and its derivatives, discarded early female deaths of newborns and risk to public health. In the initial project would be evaluated in 1200 samples of milk from 20 goat farms located in the states of Sao Paulo and Minas Gerais, considering about 30 goats per Capril. However, due to economic problems, many farmers left the activity or livestock decreased dramatically, which is hampering the harvest. The criterion for inclusion of animals per property rose to at least 20 goats per farm, considering also the minimum number of 10 properties. Our objectives are: microbiologic and biochemical characterization of coagulase positive staphylococci (S. aureus, S. intermedius and S. hyicus) in the milk of goats with mastitis, to compare the method of flow cytometry (Somacount 300 ®) used for counting somatic cell count (SCC) in bovine milk with the direct microscopic count (Prescott and Breed, 1910) and establish a correlation between the methods with the results obtained from the California Mastitis Test (CMT) which will be a screening test for subclinical mastitis; determine the presence of Staphylococcus aureus (S. aureus), Streptococcus agalactiae (S. agalactiae), and Escherichia coli (E. coli) in milk samples from lactating goats compound independent of the result of CMT and milk samples from all animals (tank), following the diagnosis by multiplex PCR (mPCR), beyond the research of other pathogens in microbiological examination and analysis of risk factors for the occurrence of mastitis, involving the laboratory test results with the variables adopted in the health management properties, obtained by applying epidemiological questionnaire. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MACHADO, GUSTAVO P.; SILVA, RODRIGO C.; GUIMARAES, FELIPE F.; SALINA, ANELISE; LANGONI, HELIO. Detecção de Staphylococcus aureus, Streptococcus agalactiae e Escherichia coli em leite caprino mastítico no Brasil, por multiplex-PCR. Pesquisa Veterinária Brasileira, v. 38, n. 7, p. 1358-1364, . (11/07107-0, 10/17254-7)
G.P. MACHADO; F.F. GUIMARÃES; B.D. MENOZZI; A. SALINA; F.S. POSSEBON; H. LANGONI. Ocorrência, patógenos e fatores de risco para mastite subclínica em cabras leiteiras. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 70, n. 5, p. 1665-1670, . (10/17254-7, 11/07107-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MACHADO, Gustavo Puglia. Caracterização microbiológica, molecular e contagem de células somáticas por citometria de fluxo (Somacount 300®) e por contagem microscopia (Prescott e Breed, 1910) do leite caprino mastítico. 2013. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.

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