Teak (Tectona grandis L. f.) has excellent physical and chemical characteristics, which ensure its high quality and durability, making it a target species of great commercial interest. However, two major concerns direct teak market: low growth rates and difficulty to activate the secondary cell formation in the stem. These questions are related to metabolic processes of wood production, involving vascular cambium cell division, cell expansion, secondary cell wall deposition, programmed cell death and compact Wood formation. Several genes are involved in these five steps, but the gene CAD (Cinnamyl alcohol dehydrogenase), (which encodes a protein that catalyzes the production of monolignols, precursors of the final lignin) has already been described in Populus trichocarpa, and shows alignments with appropriate proportions of identity (over 70%) with genes of several forest species. Indeed, the objective of this work is to characterize the CAD gene in formation and secondary growth wood tissues in teak. For the study of this gene, it will be done RNA extraction of plant material from apical buds, secondary xylem and phloem differentiation in teak individuals of different ages (5 months, one year, five years and 10 years), with the aim of isolating the gene CAD and apply molecular biology techniques and bioinformatics tools for the characterization and expression analysis in different stages of plant growth and subsequently diagnose the teak wood characteristics.
News published in Agência FAPESP Newsletter about the scholarship: