Evaluation of resin-dentin bond strenght and antimicrobial activity of adhesive systems.

Abstract

The adhesive systems development has resulted in improvement of the quality of marginal sealing and the durability of restorations. Some of them contain adhesive monomer, solvents and initiators, but additionally contain fluorides, filler particles and antibacterial compounds, which were little investigated. The objective of this study was to evaluate the resin-dentin bond strength and the antimicrobial activity of three adhesive systems. For the bond strength test, it will be used coronary dentin and the microtensile test will be conducted immediately after specimen preparation and 1 year after storage in artificial saliva. Four anaerobe microorganisms (Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum e Peptostreptococcus micros) and 4 aerobes and facultative (Staphylococcus aureus, Actinomyces naeslundii, Lactobacillus casei e Streptococcus mutans) will be used in this study. The antimicrobial activity will be determined for all microorganisms by the Inhibitory Zone, Minimum Inhibitory Concentration and the Bactericidal Minimum Concentration methods.