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Functional Characterization: The human kinase Nek5 interferes negatively in cell death and the polyglutamylation process

Grant number: 10/16831-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2011
Effective date (End): February 28, 2015
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Jörg Kobarg
Grantee:Talita Diniz Melo Hanchuk
Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovações (Brasil). Campinas , SP, Brazil


Protein kinases Nek (NIMA related kinases) are a highly conserved family of proteins involved in cell cycle progression. In humans have been identified Neks 11 (1-11), and for some of them the structure has been determined as well as the pathophysiological relevance. Several of Neks family proteins are products of genes related to diseases related to defects in cell cycle progression and the DNA repair mechanism during interphase, especially in different cancers. The Nek5 protein was identified, according to their genomic sequence in organisms such as Homo sapiens, Mus musculus, Bos taurus, among others. However, inherent characteristics of the protein remains poorly understood, as its function, structure and interactions. Therefore, the aim of this project is cloning, expression and purification Nek5 for biophysical testing and crystallization, aiming structural analysis. In parallel, will be conducted: 1) functional studies involving Nek5 silencing by RNA (iRNA) and overexpression in human cells; 2) identification of proteins that interact with Nek5 by mass spectrometry and yeast two-hybrid assay; and 3) thermal shift assays based on fluorescent and other in vitro assays to verify targets for Nek5 activity inhibition.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MELO HANCHUK, TALITA D.; PAPA, PRISCILA FERREIRA; LA GUARDIA, PAOLO G.; VERCESI, ANIBAL E.; KOBARG, JOERG. Nek5 interacts with mitochondrial proteins and interferes negatively in mitochondrial mediated cell death and respiration. CELLULAR SIGNALLING, v. 27, n. 6, p. 1168-1177, . (10/16831-0, 10/51730-0)
MELO-HANCHUK, TALITA DINIZ; SLEPICKA, PRISCILA FERREIRA; PELEGRINI, ALESSANDRA LUIZA; MARTINS MENCK, CARLOS FREDERICO; KOBARG, JORG. NEK5 interacts with topoisomerase II beta and is involved in the DNA damage response induced by etoposide. Journal of Cellular Biochemistry, v. 120, n. 10, p. 16853-16866, . (14/15982-6, 17/03489-1, 10/51730-0, 15/06458-4, 10/16831-0, 10/15262-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
HANCHUK, Talita Diniz Melo. Functional characterization: the human kinase Nek5 interferes negatively in cell death and the polyglutamylation process. 2015. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.

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