Cloning and expression of human protein kinase Nek10 structural and functional studies aimed at molecular and cellular

Abstract

The family of protein kinases NIMA (never in mitosis, gene A) was discovered in Aspergillus nidulans as a serine/threonine kinase highly conserved and important for the functioning of the cell cycle. The family of Neks (NIMA related kinases) found in mammals shows 40% identity with the kinase domain of these proteins. Thus, it plays a similar role in cell cycle progression, in general, at regions of checkpoints during interphase providing its functional relationship with motility of breast and colorectal cancer, polycystic kidney disease, among other pathologies. Protein Nek10 has not yet solved its structural features in literature, however, recently the gene loci of Nek10 was identified as susceptible to the cause of breast cancer, highlighting the importance of his study for possible treatment of this disease. For this purpose, the protein Nek10 will be cloned, expressed and purified using techniques of molecular biology to lead experiments involving the interaction, when it is phosphorylated, with other proteins and signaling pathways, and silencing its investigation of potential therapeutic targets related Nek10. (AU)