The effect of ovariectomy on dental replantation: a histomorphometric and immunohistochemistry analysis in rats

Abstract

Osteoporosis is an illness that is frequently encountered by dental surgeons in the clinic, and is caused by disequilibrium between processes of bone tissue formation and absorption. With the increasing life expectancy of the global population, more researchers are focusing on geriatrics, in order to provide the population with a healthier aging process. Individuals with osteoporosis are susceptible to both long bone and hip fractures, and dento-alveolar trauma. Considering the shortage of literature in the study of dento-alveolar trauma in individuals with osteoporosis, the aim of this study was to analyze the repair process in immediate dental replanting and its possible association with this systemic alteration (osteoporosis). A well-established experimental model, recommended by the Food and Drug Administration (FDA), was used for this analysis, i.e. ovariectomy (OVX) in rats. The project consists of two experimental groups: group I: sham rats, and group II: OVX rats. The upper right incisor of all animals will be submitted to extraction and immediate tooth replantation. Evaluation will be done by histomorphometric analysis to verify and measure external radicular resorption and immunohistochemical by the balance of proteins PCNA, RUNX-2, CASPASE-3, OPG and TRAP. (AU)