This study aims to evaluate contamination of water, sediment, vegetation and the uçá-crab by heavy metals in five mangroves of the São Paulo State, and to analyze the genotoxic impact on populations of this crustacean, based on the frequency of micronuclei cells. Mangroves are Permanent Preservation Areas (PPAs) and nurseries for many species, including the uçá-crab, which is widely marketed and consumed by humans in coastal regions. Five mangroves areas will be evaluated in São Paulo State (Cananéia, Iguape, Juréia, Cubatão and São Vicente), which was previously selected according to their level of pollution. Each mangrove will be represented by three sampling sub-areas. Sediments, tree leaves (Rhizophora mangle), corporeal structures from uçá-crab (Ucides cordatus) and water from crustacean burrows will be sampled for the quantification of six heavy metals (Cd, Cu, Pb, Cr, Mn and Hg) by atomic absorption spectrophotometry. The haemolymph of five crabs caught/subarea (n=75) will be used for the preparation of three slides/specimen and after staining they will be used to quantify the number of micronuclei cells (MCs) under microscope (1000X). Data obtained by quantification of MCs as well as heavy metal levels will be submitted to ANOVA and Nested ANOVA, respectively, which averages will be compared by Tukey's test (5%). The results obtained will assess the conservation status of mangroves and quality of natural stocks of U. cordatus, guiding actions to be leaded by government agencies for their management.
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